丛枝菌根真菌对滇重楼根茎中碳氮代谢的影响.pdf

40 1 2021 1 Vol 40 No 1 Nov 2021 doi 10 3969 j issn 1006 9690 2021 01 007 2020 07 14 2020 12 24 81260622 cstc2018jcyjAX0267 1996 E mail xulingfeng953 126 com 1991 E mail nl140828 163 com 404120 AMF AMF AMF Sdi Dh Sdi Dh S 663 1 A 1006 9690 2021 01 0043 05 Xu Lingfeng Li Zhuowei Zhou Nong Guo Dongqin Zhao Jingjing The Chongqing Engineering Lab for Green Cultivation and Deep Processing of the Three Gorges Reservoir Area s Medicinal Herbs College of Food and Biological Engineering Chongqing Three Gorges University Chongqing 404120 China Objective The content of polysaccharide and total amino acids in var derived from different arbuscular mycorrhizal fungi AMF were researched which provided a scientific basis for the screening of AMF Methods The content of polysaccharides was detected by an throne vitriol colorimetry method and the content of total amino acids was detected by chromatography Then theeffectofdifferentAMFonthecontentofpolysaccharideandtotalaminowasanalyzed Results The content of polysaccharide and total amino acids in var were significantly different due to different AMF inoculations The content of polysaccharide in var gradually increases and the content of total amino acids gradually decreases as the growth process progresses Among them Sdi and Dh significantly promoted the content of polysaccharides and total amino acids in in var Conclusion Consideringofthecontentofpolysaccharideandtotalaminoacidsin var Sdi and Dh are the most suitable alternative strains for nurturing var seedling under the experimental condition Arbuscular mycorrhizal fungi var Polysaccharide Total a mino acids 3 4 39 1 2 AMF AMF 3 4 5 6 2 4 L 8800 17 1 28 AMF 7 8 AMF AMF AMF 1 1 1 DZF UV 2450 CP225D Sartorius D 111590 200402 111591 200402 100080 200707 UV 1 2 28 AMF INVAM 1 AMF CK var 3 1 V V 2 mm 121 2 h 2013 2 24 1 mL 5 Hoagland 2013 8 2013 11 45 40 1 Table 1 Different treatment groups inoculated AMF AMF AMF Ga Rcl Gd Rin Gg Afo Gm Ako Gr Asc Sca Asp Sdi De Spe Ds Dh Ec Rco Pb Rfu Po Sde Ale Svi Atr Fm CK CK Cc 1 3 1 3 1 9 1 0 g 80 24 h 15 min 50 mL 2 0 mL 10mL 6 0 mL 4 4 1 0 8 g 400 mL 80 15 min 10mL 625nm A mg g 1 1 3 2 4 200 mg 10 mL 6 0 mol L 110 2 22 h 6 0 mol L NaOH 25 mL 1 0 mL 1 0 mL 0 02 mol L HCl 20 L 136 0 57 0 1 4 Microsoft Excel 2013 SPSS 19 0 Origin 9 1 2 2 1 1 AMF AMF Gg Gr Dh Rfu Cc 94 96 135 63 42 03 35 37 11 82 16 41 87 07 42 16 31 98 42 21 Gg Gr Dh Rfu Cc CK Gg Gr Dh Rfu Cc AMF 2 2 2 AMF Sca Sdi Spe Dh Sde Rin Ako Asp De Ds Po Ale Atr 14 61 18 97 24 47 26 13 51 12 20 07 90 58 82 66 37 00 218 43 65 65 99 09 109 28 83 36 78 90 52 05 99 03 165 77 48 35 156 12 47 56 82 31 166 22 56 81 26 55 69 69 AMF CK AMF 1 AMF Fig 1 Effects of different AMF on polysaccharide content in var 0 05 2 AMF Fig 2 Effects of different AMF on total amino acids content in var 5 4 39 2 3 3 Gd Gm Gr Cc Afo Gd Gm Gr Cc Afo CK 3 AMF Fig 3 Effects of different AMF on C N in var 3 sp 19 10 5 0 mg L 1 11 12 sp 13 AMF 5 AMF Gg Gr Dh Rfu Cc 5 AMF AMF Dixon 14 Azaizeh 15 AMF 16 Sca Sdi Spe Dh Ale At 13 AMF AMF AMF 17 AMF 4 AMF Sdi Dh Sdi Dh 1 Wen F Y Yin H X Chen C et al Chemical characteristics of saponins from var and cytotoxic activity of its main ingredient parissaponin H J Fitoterapia 2012 83 4 627 635 2 PMP HPLC J 6 4 1 2019 30 12 1503 1509 3 J 2015 31 19 149 153 4 J 2017 30 8 1760 1766 5 J 2018 37 6 1871 1879 6 J 2011 36 3 252 257 7 J 2019 38 3 615 625 8 AM J 2015 40 16 3158 3167 9 J 2014 35 14 326 329 10 sp J 2016 46 7 872 879 11 J 2015 35 2 309 314 12 J 2014 50 7 1059 1064 13 J 2016 51 17 1450 1454 14 Dixon R K Garrett H E Cox G S Boron fertilization vesicular arbuscularmycorrhizalcolonizationandgrowthofcitrusjambhi ri lush J J Plant Nutr 1989 12 6 687 700 15 Azaizeh H A MarschnerH MheldV etal Effectsofavesicular arbuscular mycorrhizal fungus and other soil microorganisms on growth mineral nutrient acquisition and root exudation of soil grown maize plants J Mycorrhiza 1995 5 5 321 327 16 D 2014 12 15 17 J 2014 45 1 23 27 7 4