低温锻炼对冷胁迫下甜瓜幼苗抗氧化酶活性与质膜透性的影响.pdf

p j,V7 P v “1,F žA q9v[6] 。Vm1 V[A,2 ℃ } /,  C M q } HW9F7  6,Œ  nbsp;T1„T2  nbsp;CKM1 ,V }  7 S, nbsp; A]} /M q  6, O2～ 3 ,T1„T2 CKAsP lt;0.01,Œ4€CKAsP lt;0.05。ƒV  } /, – nbsp;C M q   6 t ],Œ  nbsp;CKM1 , nbsp; 4C s nbsp;HW2℃ } 。  s s[7] 。m1 V[A,CKM1 ,‚] nbsp; PC  s c A†。  } , –  C  s c  } ›7/† t ],Œ nbsp; ,C  s c /† y,ƒV 4 s Z , nbsp;‚ 4 C } / s s,Q7F  s t ]。m1  nbsp; } /C M q„ s c •YFig.1 Effect of cold-hardeningon electricalconductivityand the chlorophyll contentsof muskmelon seedlingunder low temperaturestress1691 nbsp; nbsp; nbsp; nbsp;y  nbsp; } /C F Ÿ iŸ•Ym2  nbsp; } /C SOD„PODŸ•YFig.2 Effect of cold-hardeningonSOD and POD contentsof muskmelonseedlingunder low temperature stress2.2  nbsp; } /C SOD„PODŸ•YSOD、POD  V [8] 。m2 V,CKM1 ,‚] nbsp;V,C    Ÿ žA9 lt;P lt;0.01。 2℃ }/, – nbsp;T1 、T2  Ÿv/† t ],ŒSOD 、PODŸA nbsp;CKP lt;0.01。ƒV C V10 d nbsp; P8 ]Ÿ  V1T,9 lt;  }  a‹ 。Vm V[ A,T2  nbsp;rTT1 。m3  nbsp; } /C d „c •YFig.3 Effect of cold-hardening on MDA content ofmuskmelon seedlingunder lowtemperaturestress2.3  nbsp; } /C MDAc •YMDA VTK, “d s]1S[9] 。m3 V[ A,CKM1 ,10 d nbsp; PC  MDAc A 6P lt;0.01,Œ  2℃ } Hq/, nbsp;C  MDAc  –B  6 t ],ŒA nbsp;CKP lt;0.01。ƒV 10 d nbsp; –„ C  B V,Œ 9 lt;  }  a‹ 。3 结论与讨论V[ s V[ A,‚] nbsp;C  s  T,Q7F  s,P  2 ℃ } /c  A†。 “} HW,C SOD、PODc  /† t ],MDAc „M q5  6t ]。Œ  nbsp;CKM1 ,‚] nbsp; A] } /C   s[MDA„M q  6。 s s 3 – “v •Y,Œ ;† Ž/†€B„。7  s„MDAM q  6]5B h  ]C  T。 iŸ„ s c  ™  3
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lt;。V , I iŸ„ s c  ‚  3Z_Z[ 6,7] 。39; 10 d nbsp; A]C  iŸ  } 9v,Œ nbsp; s B T,yN†V s c Z ƒ, nbsp;C ‚ •Y。Œ  nbsp; A4C SOD、PODŸ,]C  170  nbsp;j lt;   nbsp; nbsp; nbsp; nbsp; nbsp; nbsp; nbsp; nbsp; nbsp;16 MDA 3„M q  6。7SOD、POD} / FeŸT[1,2,6～ 10] ,MDA V1, cM qQ˜“  [11] 。yN,VƒtSM f ƒ Ÿ A, nbsp; †C  Ÿ, P FeŸž4。 6“,VPODŸM Ÿ A,‚] nbsp;C FeŸ 4‚],7†  nbsp;F†€B„。• ID[1] nbsp; ,Š1, nbsp;,. ;} CQ1 tc •Y[ J] .  jlt;v1 – S,2005,36167～ 69.[2] nbsp;, nbsp;.} CQ F I 3 S[ J] .Z\,2006,123～ 24.[3] nbsp;e 7, Siž, ,. aR nbsp;4  „I N F Ÿ  3 [ J] .T, 2005,3108971～ 976.[4] nbsp; † 3. 3 3 L „/ Œ[ M] .,2000.164～ 165,167～ 169,260～ 261,261～ 263.[ 5] nbsp; m,R,Z ,.  s c ZE[ J] .S jY,2005, 6153～ 155.[ 6] nbsp;
, ,’. R F Ÿ 3 3Ss[ J] . jlt; S,2006, 2211～ 213.[ 7] nbsp; ] ,,S, . ;  ;/ i 0ŸS[ J] . jlt;, 2005, S273～ 75.[ 8] nbsp;,, j.} / V Ÿ‘„  ŸM[ J] .\, 2005, 3205911～ 913.[9] nbsp;,fS\,ˆ ,. ; œˆ F Ÿ iŸ•Y[ J] .,2005, 25122478～ 2483.[ 10] nbsp;ˆ ;,,Rš .,.} Q „1   Ÿ•Y[ J] .  jlt;v1 – S,2002,333302～ 304.[ 11] nbsp;Larc D A, Tottempudi K P, Cecil R S .Changes in isczyme profiles of catalase, peroxidase, and glutathione re-ductase during acclimation to chilling in mesocotylsof maizeseedling[ J] .Plant Physiol, 1995, 109 1247～ 1257.上接第99页• ID[1] nbsp;I L,\ ,.SPh]C_f]yp[ J] ._f,1996,101233～ 236.[2] nbsp; 2.€ oh  ][ J] .S_,1996,231～ 34.[3] nbsp;,u *, .B„RT-PCT_ 2’hZEy [ J] ._f, 2003,176330～ 332.[ 4] nbsp;Wetzel T, Wegener U, et al .Complete nucleotide sequenceof the RNA1 of a grapecine isolate of Arabis mosaic virus[ J] .Arch Virol, 2004,149989～ 995.[ 5] nbsp;Wetzel T, Meunier L, Jaeger U, et al .Complete nucleotidesequence of the RNAS2 of German isolates of Grapevinefanleaf and Arabis mosaic nepovirues[ J] .Virus Research ,2001,75139～ 145.1711 nbsp; nbsp; nbsp; nbsp;y  nbsp; } /C F Ÿ iŸ•Y/p