低温锻炼对冷胁迫下甜瓜幼苗抗氧化酶活性与质膜透性的影响.pdf
<p> j,V7 P% =ȳÉv 1,ÁF®Ï AÈ q9v6 。Vm1 VA,2 ¥ Ï ç/, îµ) Ø¥C(¼ ìMÈ q ( Û Ï ç HW¥9F7 6, Ü®Ñ ÿ¥T1T2) ØÐÃÜ ÿ¥CKM1 ,V Ï ç¥»=? 7 S,®Ñ ÿ ? üAÅÏ ç/MÈ q¥ 6, O»2 3? =,T1T2) Ø (ÐCKµA÷µs(P <0.01),»4?=ÐCKµA÷µs(P <0.05)。V ü Ï ç/, Ù ®Ñ ÿ¥C(¼ ìMÈ q ¯ï 6 t , ÃÜ ÿ¥CKM1 ,®Ñ ÿ ?4ÚC(¼ ì § s HW2¥ Ï ç ? ï。É ù®ÑöÁ= s Ís³7 。®m1 VA,ÐCKM1 ,ñ¥®Ñ ÿ ? PC(¼ ì¥= s Íc A÷®。 Ûª¥ Ïç) ØÏ, Ù îµ¥) ØC(¼ ì= s Íc ( Û Ï ç¥É7ï/ t ,®Ñ ÿ¥) Ø,C(¼ ì= s Íc /¥÷ y,V ü4Ú= s ÍZ ë,®Ñ ÿ ?4Ú ¹C(¼ì Ï ç/= s Í¥s³,Q7µF s³¥ t 。m1 ®Ñ ÿ Ï ç/C(¼ ìMÈ q= s Íc ¥YFig.1 Effect of cold-hardeningon electricalconductivityand the chlorophyll contentsof muskmelon seedlingunder low temperaturestress·169·1 ù Ùy ü©:®Ñ ÿ Ï ç/C(¼ ì FÄ ½ÐÉ i¥Ym2 ®Ñ ÿ Ï ç/C(¼ ìSODPOD¥YFig.2 Effect of cold-hardeningonSOD and POD contentsof muskmelonseedlingunder low temperature stress2.2 ®Ñ ÿ Ï ç/C(¼ ìSODPOD¥YSOD、POD© ±þ «VÄ¥ ¹½8 。®m2 V©,ÐCKM1 ,ñ¥®Ñ ÿVñÏ,C(¼ ì¥ Õ ¹ ½ (¤A÷9 <(P <0.01)。 Ûª2¥ Ïç/, Ù Ü®Ñ ÿ¥T1 、T2) Ø¥ ½ïïv/ t ,SOD 、POD¥A÷Ú¿ÃÜ®Ñ ÿ¥CK(P <0.01)。V üC(¼ ìÜV10 d®Ñ ÿª ?P8 =Þ ðVÄþ1®¥ÅT¨,9 < Ï çÌ⥠a ? ï。VmÏÎ V A,T2) Ø¥®Ñ ÿrTª¿T1) Ø。m3 ®Ñ ÿ Ï ç/C(¼ ìd= c ¥YFig.3 Effect of cold-hardening on MDA content ofmuskmelon seedlingunder lowtemperaturestress2.3 ®Ñ ÿ Ï ç/C(¼ ìMDAc ¥YMDA «VÄT¨¥KÔÁþ, “d s¥×1S½9 。®m3 V A,ÐCKM1 ,10 d®Ñ ÿ ? PC(¼ ì= ËMDAc A÷ 6Ú(P <0.01), Ûª2¥ Ï çHq/,®Ñ ÿ¥C(¼ ì= ËMDAc Ù B°ï 6 t ,A÷®¿ÃÜ®Ñ ÿ¥CK(P <0.01)。V ü10 d¥®Ñ ÿ Ù ö C(¼ ì= ËBçñ¥ «VÄ, ?9 < Ï ç¥ a ? ï。3 结论与讨论V s V A,ñ®Ñ ÿC(¼ ì= s Í Àµ ¹T¨,Q7F s³,P Ûª2 Ï ç/¥c üA®。 Û“Ï ç HW¥üÉ,C(¼ ìSOD、PODc (ï/ t ,MDAc MÈ q5ï 6t 。 ÐÃÜ ÿ¥CKM1 ,ñ®Ñ ÿ ?A÷Å Ï ç/C(¼ ì ¹ ½¥s³#MDAMÈ q¥ 6。= s Í¥s³±é 3É?â Ù µv¥µ ëY, ; ? ï /µÉBùî。7 ¹ ½s³MDAÐMÈ q 6¥Å5Bçñ h ÏC(¼ ì¥ åÊT¨。% i= s Íc û ±é 3 ï¥×1 3 Ø·S,Bî% i®,= s ÍcÚ5±é 3 ï <。ùîV ü, Iâϱé% i= s Íc (Ô æ¿±é 3É?â¥Z_?Z 6,7 。'ùî ª¹10 d®Ñ ÿ ?A÷ÅC(¼ ì% i Ûª Ï çÌâÏ¥9v,®Ñ ÿ= s Í µBç¥ åÊT¨,yNV= s Íc Z ë,®Ñ ÿC(±é µ ¥Y。 ®Ñ ÿ ?A÷4ÚC(¼ ìSOD、POD,ÅC(¼ ì= ËÏ·170· j < Ð 16 MDA¥ 3îMÈ q¥ 6。7SOD、POD®Ñ ç/±é Feµ¥T¨1,2,6 10 ,MDA «VÄ¥ö1Áþ, cÐMÈ qQ“É ¥ å ñ11 。yN,Vt·S¥MÄ f A,®Ñ ÿ ?®C(¼ ì±é®Ñ¥ ùË, P±é Fe¤4Ú。 6,VPOD©¥MÄ A,æ®Ñ ÿC(¼ ì Fe ?¥4Úñ,#7 Ø®Ñ ÿ¥F¶ÉBùî。 IÓD:1 ìÖ ¼,1Ú,¦ ,©.®Ñ Ð ç©ÕC(Q¤ìÐ1ô ì tþÉc ¥Y J . öê j<vÐÐ(1 SÐñ),2005,36(1):67 69.2 É¡,Ú .®Ñ çC(Q¤ ì F I 3 Ø·S¥ùî J .Zó,2006,(1):23 24.3 e 7î, Si, Ù¡,©. aR®Ñ ÿ4Ú Ï ù˱þÖ ÜI N¥ F Ï# 3 Ø$ J .TþÐ, 2005,31(08):971 976.4 Ù 3.±þ 3 Ø 3Ä Lð Ø/ M .Ø:Ú©âñ,2000.164 165,167 169,260 261,261 263. 5 Ù¤m,R),ôZ ,©.Ö Ü= s Íc ©çZEùî J .ÏS jÐY,2005, (6):153 155. 6 ·É, Ù=,Æ¿.° R F Ï 3 Ø 3Ä·Ss J .µ j< SÐ,2006, (2):211 213. 7 ç ,S,¦ é. ÐÐ Ê®Ñ Ð/Ñ iÛ( 0·S¥ùî J . j<ýñÐ, 2005, (S2):73 75. 8 ç¶,¥øê,à j.®Ñ ç/Éöø¼ ì V ·âÉ% ¹ ½¥MÄ J .óÐ, 2005, 32(05):911 913.9 Ù?¿,fS, Á,©.®Ñ Ð ¼ ì FÄ ½ÐÉ i¥Y J .±þÐ,2005, 25(12):2478 2483. 10 ;,¿:Ä,R .,©.®Ñ çÛ(Q¤ ì1ô ì ¹ ½¥Y J . öê j<vÐÐ(1 SÐñ),2002,33(3):302 304. 11 Larc D A, Tottempudi K P, Cecil R S .Changes in isczyme profiles of catalase, peroxidase, and glutathione re-ductase during acclimation to chilling in mesocotylsof maizeseedling J .Plant Physiol, 1995, 109 :1247 1257.(上接第99页) IÓD:1 I üL, á ,.áSÙÁPhùîC#_fÍyp J .±þ_f,1996,10(1):233 236.2 ç¥ 2. § $ oÔ½ñhýÕ Ë# Í J .ÏSÉâî±_,1996,(2):31 34.3 Ô¿Ð,u® *,Û½ Ü.BRT-PCT_© 2·½=hýZE¥y ë J .±þ_f, 2003,17(6):330 332. 4 Wetzel T, Wegener U, et al .Complete nucleotide sequenceof the RNA1 of a grapecine isolate of Arabis mosaic virus J .Arch Virol, 2004,149:989 995. 5 Wetzel T, Meunier L, Jaeger U, et al .Complete nucleotidesequence of the RNAS2 of German isolates of Grapevinefanleaf and Arabis mosaic nepovirues J .Virus Research ,2001,75:139 145.·171·1 ù Ùy ü©:®Ñ ÿ Ï ç/C(¼ ì FÄ ½ÐÉ i¥Y</p>
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