低浓度NaHSO3处理对温室黄瓜光合作用的影响

<p>·$ùî·® iNaHSO3) ØÑ iÛ(;T¨¥Y¢mu1, 2, Ù Wô2, &nbsp;Î3,¦ 4(1.安徽农业大学园艺学院, 安徽合肥 230036;2.上海市农业科学院, 上海市设施园艺技术重点实验室,上海 201106;3.上海交通大学农业与生物学院, 上海 201101;4.上海市宝山区生物技术中心, 上海 201907)K 1:¹)® ið ; Ö÷¿Û(;T¨¥Y# Å,Ñ i ¡Hq/,Û( ÖÕDeltastar¹ k,¨® i¥NaHSO3 ·A ¨ ) Ø 3â ù¥Û(= Ë。²TV ü:® iNaHSO3 V4ÚÑiÛ(= Ë¥ Î q。Ñ iÛ(NaHSO3) Ø¥ aR i¹2.0 mmol/LP·,Û( ì ù 7½²( ù ¨® iNaHSO3 ·A ( V4ÚÛ(= Ë¥ Î q,i ?ûÃT¨3 dP·。Ðv( ¨ £)M1,2.0 mmol/LNaHSO3) Ø4ÚÛ(ÿ ?= Ë Î q20 %P·, OÐX©¥ÉÖÌÖÄ¥£ùy0PMS¥T¨ Ë », 9F ATP¥,7idY= Ë= s ÍPS¥ÄÐr q。ÓcÎ ! =;vf /NaHSO3¥¨É ) 。1oM:Û(;Ñ i;T¨NaHSO3Ïms Ë|:Q945.11ÓDS M :AÓcI|:1007-7146(2009)03-0295-05EffectsofLowConcentrationNaHSO3 TreatmentonPhotosynthesisofCucumberinGreenhousePEIXiao-bo1, 2, LIShi-cheng2, CAIRun3, WANGYue4(1.SchoolofHorticulture, AnhuiAgriculturalUniversity, Hefei230036, Anhui, China;2.ShanghaiAcademyofAgriculturalScience, ShanghaiKeyLaboratoryofProtectedHorticulturalTechnology, Shanghai201106, China;3.CollegeofAgronomyandBiology, ShanghaiJiaotongUniversity, Shanghai201101, China;4.CenterofBiotechnologyofBao-shan, Shanghai201907, China)Abstract:Toprobetheinfluenceandmechanismoflowconcentrationofsulfiteonphotosynthesisofcucumber, onecu-cumbervariety“Deltastar” waschosenandsprayedwithNaHSO3 atleavesinlowconcentrationatdiferentstages, theresultsindicatedthatthephotosynthesisrateofleaveswasimprovedingreenhouse.Theoptimalconcentrationofcucum-berwasabout2.0 mmol/L.Thetreatmentwaseffectivewhencucumberatplantletoratblooming fruitingstage, withcontinuouslyeffectmaintainedforabout3 days.Comparedtothecontrol(sprayingwater), thephotosynthesisrateofcucumberfunctionalleaveswasimprovedby20 %with2.0 mmol/LNaHSO3 treatment, inaccordwithPhenazinemet-hosulfate(PMS)whichisacofactorofcatalyzingcyclic-photophosphroylation, raisingtheATPcontentintheleaves.AndthepotentialphotochemicalefficiencyofPS wasnotafectedbysprayinglowconcentrationofNaHSO3.Applica-tionofNaHSO3 intoprotectedcultureunderinsuficientlightconditionwasalsodiscussedinthearticle.»18 »3 ù2009 M6 &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; ; &nbsp;3 þ Ð ACTA LASER BIOLOGY SINICA &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Vol.18 No.3Jun.2009 là ° ù:2008-09-15;©í ° ù:2009-01-10Á “:SE S/¦ê9à “(2008BADCOB02);SE1 SÐÁ×Ä “(39830230);½ê 81 SÐÁ “(03041106);½ê 8ª® ¦ ¡! “Teº:(1970), 3,½êÈ a ¦,¬ q,p V,ö1ùîZ_¹ ! ó。(È0¯Q)peixiaoboahau.edu.cn;peixbsohu.comKeywords:cucumber;greenhouse;photosynthesis;Sodiumbi-sulfite(NaHSO3)Tþ ¨ ÒNaHSO34Ú ;T¨9FÁ ¥÷EáSXÜ k¨ 1-3 ,µ¬î¹BñN÷、í、µr7 j&lt; 3ÁÏ Vw&lt;¨¥zn 。* ù¥ùî ª¹ 4ÚT¨¿NaH-SO3Å ±þ« ¥ø#,i| T¹B Ë« Å4 P¨。/ ¥ Ø $ùîVü 4-9 ,® iNaHSO3;T¨¥Ér¿ñYVɶ ¡PSI¥ÖÌÈ0.¾# Ò ó¥ÖÄ,9FATP¥,i ®¿ ñÅ ;« ,É d 7,T¹ 2¥ 3É?â# îA³¥ ;g7 T¨¥。;T¨ ±þK×1¥ 3 ØVñ,9 ±þîÄþÉÐÁ ¥$。Tþ NaHSO3)Ø i# rTXµ 10-12 , ÂÇÍ ¨5 mol/LNaHSO3 ?h &nbsp;ù®Ñ#;T¨¥Y, 100 mg/LNaHSO3µr¹4Ú$ ö Ë Ñ=Ë ä Í¥c , m 3¯ ¨200 mg/LNaH-SO3 ?4Ú Î q= s Íc , P¯=9Á4Ú ÖÉ。2 mmol/LNaHSO3 V Pl . ìÁ 9F, P b ÖÎð ? ïh Ð,KÔÁ. ìâÉc/。YVNaHSO3) ØÑ iÛ(¥T¨Y# Å¥),+Y ! =;v f /NaHSO3) Ø ?ÉÑ iÛ(¥ Î q, ?¹ 3Á ¥¨4G &nbsp;。LÏ,¨É ¡!A9à 1î“d eÅ, £ LVñÏÛ(±é 3âBÁ。 æ¨Ñ i = ,Ç ? LC kÏ ÐÌ⥠eÅBÁ×ç,7 O VC L= 3ÁÏ¥v¥ f。1 ÐZEL ÖÕ¹Û(CucumissativusL.), §Ñ i¨ ÖÕz(Deltastar, RUKZWAAN ³)。 L¿2005 M Z j&lt; SÐýj ðÑ iÏÉ。L¨ èû ¡,È?5 Ø。 Û uF !9, Äl u30é, 3Qׯ。NaHSO3) ØsY¿Û( ìù、 7½²( ù±é ¥= Ë ¨ ÒNaHSO3。¿ d?Ê4É 、=ÊBÁ¥î = ˨ѱ ¨üÉ ¨ü) Ø, ¨ © Ö0 £¹v(CK)。;T¨¥ &lt;Y wL©ç¨Li-6400;N。NaHSO3) تÛ(= Ë Î q¥BW©ç¨Li-6400;N©ç。ATPc ¥©çvÓD 13¥ZE,Ê | §ë¥Û(î ÿ ?=,5|Ï “,Ï §¥ö=sYTv( ¨ Ö0 £)Ð) Ø( ¨2 mmol/LNaHSO3),¿Q °g | MÊ、 ëM©¥= Ë ,m £) Ø( ê µi4 |)10 min, Ï ª, |Bç8 ·A¨ Í ½E©ç Ï¥ATPc 。= s Í ¥©ç:¨PAM-2100Lz TØÅN(Walz, Efeltrich, Germany)。 æ¨ ¥!± 0/Ñh¥=C2030-BCþ= Ë,¿M=Ê= Ë©ç = s Í 。© çÂ/:ÜV s20 minÀ a¥= Ë,¨B l¿0.1 mol/m2·s¥© ;( Ô q¹600 Hz)v ,¤ S;F0;u 7Bñ ;(8 000 mol/m2·s, 20 KHz, 0.8 s),¤À a/¥Kv;Fm;PSI¥Kv;ÄÐr qFv/Fm¨/ T9 Ø:Fv/Fm=(Fm-F0)/Fm。 Û©ç6é,ׯ3Q。2 ²TÐs2.1 NaHSO3) ØÑ iÛ(= Ë Î q¥Y¨ iNaHSO3 ¨ ÒÑ iÛ(±é= Ë,Q° ö1 = Ë¥ Î q(m1), TÐl ¥ùî²TM »,) Ø= Ë¥ Î q(Pn) üAÚ¿v,Ñ iÛ( NaHSO3¥ aR i¹2 mmol/LP·。1 2 mmol/LNaHSO3) Øv= Ë¥T¨Y wL(m2), VnNaHSO3)ت, = Ë/ Ð/¥ Î q (¤É。vBHq/sY¿Ñ iÛ( ì ù 7½²( ùÛ(= ËÉ2 mmol/LNaHSO3) Ø,¥ &lt;Hq/(m3) ( Vµr4Ú Î q20 % ,NÉT¨ VûÃ3 dP·(m4)。2.2 NaHSO3PMS) ØÛ(= Ë Î q¥Y±þ= ËÏy;ÖÄ4ATP ? ¡Ä³17î¹T¨¥KÅy0,|bÄÖÌÖÄ¥£ùy0 ; ÖJq(PMS)Æ= ËÈ V9F= ËÏATPc 4Úr q。296 &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; ; &nbsp;3 þ Ð &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; »18 Û( 7½ ù,¨2.0 mmol/LNaHSO3) Ø¥Û(= Ë,»2?10:00©ç¥ATPc (mol/m2)¹5.12 ±0.37,7vϺµ3.01 ±0.23(P=0.033,µsA÷)。1 2.0 mmol/LNaHSO30.2mol/LPMSsY) Ø ) ØÛ(= ËÎ q¥Y(m5),²TV ü, ñ ÌsY) Ø ) Ø ( VÉ Î q20 %P·,sY) ØÐ ) Ø î4Ú Î q¥ñM »,V ü ñ Ì¥ÉT¨ ( V ? 9F ATP¥î,y7 ) ØÃVCßFr。|bÄÖÌÖÄ¥£ùy0 ; ÖJq(PMS) Æ= Ë V9F= ËÏATPc 4Úr q。297»3 ù &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; ¢mu©:® iNaHSO3) ØÑ iÛ(;T¨¥Y &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;NaHSO3处理浓度为 2.0 mmol/L, PMS浓度为 0.2mol/L,测定光强为 1 000 mol/m2.s。Treatedwith2 mmol/LNaHSO3 and/orconcentrationofPMS0.2 mol/L, measuredwithlightintensityof1 000 molphotons/m·s.图 5 花期NaHSO3和PMS处理对黄瓜叶片净光合速率的影响Fig.5 TheeffectofNaHSO3 andPMSonnetphotosynthsisrateincucumberleafatbloomstage2.3 NaHSO3) ØÛ(= Ë= s Í¥Y= s Í Fv/FmT¹ PS¥×1; , ñQPS¥ÄÐr q。 Âm6 îU, 0.5 mmol/L 4.0 mmol/LNaHSO3) ØÑ iÛ(= s ÍFv/FmYA÷,V üPSid®iNaHSO3) ØY¥ÊÄ。CK, A, B, C, D, E分别表示对照, 0.5 mmol/L, 1.0mmol/L, 2.0 mmol/L, 3.0 mmol/L和 4.0 mmol/LNaHSO3处理。CK, A, B, C, DandErepresentcontrol, andtreatedwithNaHSO3 atconcentration(mmol/L)of0.5, 1.0, 2.0.3.0and4.0 respectively.图 6 黄瓜花期 NaHSO3处理对 PS光化学效率(Fv/Fm)的影响Fig.6 TheefectofNaHSO3 onthepotentialphotochemi-caleficiency(Fv/Fm)ofPS incucumberatbloomstage3 ) Tþ ¨ ® iNaHSO3É Î q¥T¨, Ö8 L8 = LÏ (¤£ L 4-6 ,NaH-SO34Ú Î qô Û“ ñÉ= Ë =¥ÖÄ,9F = s8îATP¥ ? ï,ö1By¿ ñÉ ¶ ¡PS¥Ì TÈ0.¾ 9 。 - ¦ùî ª¹ 14-15 ,±þ= ËÏy;ÖÄ4ATP ? ¡Ä³17î¹T¨¥KÅy0,|bÄÖÌÖÄ¥£ùy0 ; ÖJq(PMS) Æ= ËÈ V9F= ËÏATPc 4Úr q。 LϨNaHSO3X© VÉÖÌÖÄ¥£ùy0PMS ) ØÛ(= ˥Πq4ÚíßFr,£ L NaHSO3) Ø= Ë4Ú Î q¥ðyö1 É ;ÖÄ9FATP¥ø#。¿ 2Z ! 3Á7ý,+Y CÑ i,ë=1«Ñ i =K®ÑBî»û20 P·,ÜÈC;v õ|Ï? ,;v Û(¥Ä(Á v/,ªªî¹ Kö1¥KÅy0 16 。V&#39; L¥²T A,Ñ i 3ÁÏí Û(ì ùÎ 7½²( ù,= Ë ¨ Ò2 mmol/LNaHSO3(µrT, V4Ú ; Î q20 %P·Ñ i =¥v(PAR) Ú®,® iNaHSO3) Ø (µrT, OPAR®¿200 mol/m2·s H,NaHSO3) Ø4Ú= Ë Î q¥rT÷F üA(m3、m4)。yN, ! 3ÁÏ ¨ Ò® iNaHSO3Ç ?4Ú = Ë Î q,7 O¹Ï³h ! =;v 3Á ¥µ ëY4 BHoå。References 1 ¸ÖP,B Y.ð ; Ö _ ) N0 3É?â¥Y J.j&lt;Ð/ , 1993, 4:30-32.XUYu-xiang, TIANShu-fang.EfectofNaHSO3 onGrowthandDevelopmentofEggplant J.AgronomyandTechnology,1993, 4:30-32. 2¦ld,ñkê.ð ; Ö _ )$ ö Ë Ñ ¥¨ùîJ.T SÐ, 1991, 8(2):97-99.WANGYi-zhang, WUXiao-dong.AppliedResearchofSodiumBisulfiteonDangshanPear J.FruitScience, 1991, 8(2):97-99. 3p. , O.ð ; Ö _ ) m 3¯Á Ð ÖÉ¥¿ ÿJ.ÏS jÐY, 2000, 16(4):15-17.TUChuan-lin, KETan.ImprovementofTeaYieldandQualitybyNaHSO3 inHilAreaJ.ChineseAgriculturalScienceBulle-tin, 2000, 16(4):15-17. 4L, + Ñ.ð ; Ö _ );# ¥YJ.±þ 3 ØÐ, 1987, 13:42-50.298 &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; ; &nbsp;3 þ Ð &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; »18 TANShi, SHENYun-gang.TheEfectofSodiumBisulfiteonPhotosyntheticApparatusandItsOperationJ.Phytophysiologi-ca, 1987, 13:42-50. 5ÉE å, + Ñ, Ù£2,©.ð ; Ö _ )® &lt;/= s8ÖÌÖÄ¥ÉT¨J.±þ 3 ØÐ, 1989,15:101-104.WEIJia-mian, SHENYun-gang, LIDe-yao, etal.StimulatoryEfectofCyclic-photophosphorylationofChloroplastsunderLowLightIntensity J.ActaPhytophysiologicaSinica, 1989, 15:101-104. 6¦¡Í,ÉE å. ¨ Ò® ið ; Ö _ ) VÉl = ËÖÄT¨J. 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