臭椿等20种植物粗提物对烟蚜的杀虫活性.pdf

Chinese Tobacco Science 2020 41 6 76 81 DOI 10 13496 j issn 1007 5119 2020 00 131 20 1 1 1 2 1 1 1 650224 2 674800 20 48 83 mg L Equisetum ramosissimum Dicranostigma leptopodum Ailanthus altissima 24 h 50 53 33 56 67 74 67 20 LC50 Datura stramonium LC50 26 618 29 179 41 484 87 741 mg L 100 mg L Study on the Insecticidal Activities to Myzus Persicae of Extracts from Twenty Plant Species LIANG Qian 1 LI Chunyan 1 SHAN Zhengwei 1 QIN Zhaoxi 2 HU Yong 1 LIU Weiyi 1 1 Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China Ministry of Education Southwest Forestry University Kunming 650224 China 2 Yunnan Tobacco Company Lijiang Branch Lijiang Yunnan 674800 China Abstract In order to screen plant resource with significant insecticidal activities against M Persicae the effects of laboratory bioassays of 20 plant extracts to M persicae were investigated by the immerse leaf method The results showed that when the concentration was 48 83 mg L the 24 h corrected mortality of the extracts from Equisetum ramosissimum Dicranostigma leptopodum and Ailanthus altissima were 53 33 56 67 and 74 67 respectively It was found that 20 plant extracts exhibited various levels of insecticidal activities against M Persicae The pesticidal activities of A altissima D leptopodum E ramosissimum and D stramonium were significantly higher than that of other tested extracts and the LC50 were 26 618 29 179 41 484 and 87 741 mg L respectively The results provide theoretical basis for biological control of M persicae and scientific utilization of these 20 plant resources Keywords plant extracts Ailanthus altissima screen Myzus persicae Myzus persicae Sulzer Homoptera Aphididae Myzus 1 400 CMV Y PVY TEV 2 3 4 5 6 7 8 9 10 11 12 13 31201572 111307 1975 E mail liangqian533 E mail weiyiliu651 20 20 0 4 3 0 2020 06 01 6 20 77 7 14 15 16 17 18 19 2400 20 30 40 20 21 20 1 1 1 1 1 1 2019 6 7 1 1 1 2 1 2 1 2 1 20 95 3 30 min 1 Table 1 Samples of tested plants No Plant names Latin name Sample origin Collect parts 1 Dicranostigma leptopodum Maxim Fedde 2 Buddleja officinalis Maxim 3 Catharanthus roseus L G Don 4 Euphorbia helioscopia L 5 Eriobotrya japonica Thunb Lindl 6 Photinia serratifolia Desfontaines Kalkman 7 Ligustrum quihoui Carr 8 Camptotheca acuminata Decne 9 Nandina domestica Thunb 10 Equisetum ramosissimum Desf 11 Girardinia diversifolia Link Friis 12 Polygonum perfoliatum L 13 Tribulus terrestris Linnaeus 14 Xanthium strumarium L 15 Datura stramonium L 16 Ailanthus altissima Mill Swingle 17 Ricinus communis L 18 Cinnamomum camphora L presl 19 杧 Mangifera indica L 20 Sophora flavescens Ait 1 2 2 50 mL 0 5 80 50 g L 0 5 80 1 2 3 22 20 5 6 7 8 2020 41 10 5 s 22 9 cm 25 1 65 5 L D 16 h 8 h 24 h Abbott 20 100 1 100 1 2 4 SPSS 23 0 Anova 20 LC 50 LC 50 95 2 2 2 1 20 2 390 63 mg L 杧 24 h 50 64 00 60 67 67 67 64 33 195 31 mg L 杧 24 h 50 60 67 58 67 67 67 53 33 64 00 85 33 53 33 48 83 mg L 24 h 50 56 67 53 33 74 67 0 5 80 24 h 7 4 mg L 24 h 90 2 2 20 2 LC 50 LC 50 95 3 3 20 24 h LC 50 26 618 mg L 19 24 h LC 50 29 179 41 484 87 741 mg L 杧 LC 50 100 1000 mg L 24 h LC 50 2 167 433 mg L 3 20 LC 50 26 618 mg L 1 6 24 h LC 50 11 460 mg L 48 h LC 50 4110 mg L 23 10 24 h LC 50 258 90 mg L 24 24 h LC 50 157 73 mg L 25 LC 50 206 39 mg L 26 27 28 29 30 31 32 6 20 79 2 20 24 h Table 2 Corrected mortality of twenty plant extracts against M persicae in 24 hours Crude extract Concentrations mg L 1 Corrected mortality Crude extract Concentrations mg L 1 Corrected mortality 24 41 46 00 6 35a 195 31 42 67 9 39a 48 83 56 67 12 41a 390 63 46 00 6 35a 97 66 57 00 11 00a 781 25 53 33 3 67a 195 31 60 67 7 33a 1 562 50 57 00 6 35a 390 63 64 00 3 67a 3 125 00 62 33 11 05a 48 83 31 67 3 33b 390 63 35 33 6 06c 97 66 38 67 3 67a 781 25 36 67 1 67b 195 31 42 33 3 67a 1 562 50 48 33 2 33b 390 63 49 67 9 70a 3 125 00 53 67 9 96ab 781 25 49 67 3 67a 6 250 00 70 33 3 93a 390 63 31 67 3 33c 12 21 30 67 10 33b 781 25 35 33 6 06c 24 42 36 67 6 17a 1 562 50 38 00 4 04bc 48 83 39 67 7 31a 3 125 00 53 33 7 33b 97 66 42 33 3 67a 6 250 00 70 33 3 93a 195 31 58 67 6 33a 390 63 31 67 3 33c 390 63 33 33 4 41c 781 25 33 33 4 41c 781 25 38 67 3 67c 1 562 50 38 00 5 29bc 1 562 50 49 67 3 67b 3 125 00 56 67 10 67b 3 125 00 56 00 1 00ab 6 250 00 86 67 2 33a 6 250 00 65 33 2 67a 390 63 35 33 6 06b 12 21 31 67 3 33c 781 25 54 67 10 14a 24 42 46 00 6 35b 1 562 50 63 33 4 91a 48 83 53 33 9 70ab 3 125 00 67 00 5 51a 97 66 60 67 7 33ab 6 250 00 74 33 7 84a 195 31 67 67 6 06a 97 66 35 33 6 06b 390 63 46 00 6 35c 195 31 38 67 3 67b 781 25 49 67 3 67b 390 63 39 00 7 00b 1 562 50 53 33 3 67ab 781 25 46 00 6 35b 3 125 00 60 67 3 67ab 1 562 50 57 00 6 35a 6 250 00 64 33 3 67a 48 83 31 67 3 33c 195 31 31 67 3 33b 97 66 42 33 3 67b 390 63 35 00 0 00b 195 31 53 33 3 67ab 781 25 42 33 3 67b 390 63 60 67 7 33a 1 562 50 42 67 3 84b 781 25 64 33 3 67a 3 125 00 64 00 7 00a 48 83 38 67 3 67b 12 21 31 67 3 33b 97 66 53 33 7 33b 24 42 42 00 2 00b 195 31 64 00 7 00b 48 83 74 67 6 67a 390 63 67 67 6 06b 97 66 74 67 3 33a 781 25 71 33 3 33a 195 31 85 33 3 67a 390 63 38 67 3 67b 195 31 42 33 3 67b 781 25 46 00 6 35a 390 63 49 67 3 67b 1 562 50 57 00 6 35a 781 25 53 33 7 33b 3 125 00 58 67 10 71a 1 562 50 57 00 6 35ab 6 250 00 65 67 2 33a 3 125 00 76 00 11 00a 杧 48 83 42 33 3 67c 390 63 38 67 3 67b 97 66 46 00 6 35c 781 25 44 33 4 33a 195 31 53 33 3 67bc 1 562 50 46 00 6 35a 390 63 64 33 3 67ab 3 125 00 55 33 6 57a 781 25 71 33 3 33a 6 250 00 57 00 6 35a 0 25 53 33 3 33b 0 50 73 33 3 33a 1 00 76 67 8 82a 2 00 83 33 8 82a 4 00 90 00 5 77a p 0 05 Note Different letters in the same column after Corrected mortality SE mean significant differences p 0 05 19 33 34 35 C 20 C 19 C 22 C 25 20 34 36 8 0 2020 41 3 20 Table 3 Contact toxicity of twenty plant extracts to M persicae Plant names Toxicity equation LC 50 mg L 1 95 95 FL Slope SE Chi aquare 2 0 340 x 0 498 29 179 0 425 65 366 0 340 0 133 1 018 0 412x 1 136 570 936 197 025 1 100 323 0 412 0 133 0 108 0 402x 1 110 577 344 291 635 5 503 087 0 402 0 134 0 623 0 745x 2 412 1 718 106 1 200 658 2 526 551 0 745 0 136 2 116 0 812x 2 710 2 167 433 1 575 665 3 215 140 0 812 0 137 4 832 0 526x 1 099 123 333 73 007 382 282 0 526 0 135 2 577 1 200 x 3 843 1 592 520 163 061 2 8781 950 1 200 0 143 18 366 0 692x 2 245 1 764 714 1 199 659 2 700 415 0 692 0 135 0 199 0 792x 2 294 790 754 474 890 1 114 989 0 792 0 138 3 197 0 746x 1 207 41 484 27 884 59 030 0 746 0 136 0 721 0 434x 1 299 981 416 530 901 5 097 130 0 434 0 134 1 581 0 397x 1 146 768 263 141 017 1 460 765 0 397 0 133 0 244 0 708x 1 612 188 844 126 926 277 899 0 708 0 135 0 995 0 619x 1 967 1 505 145 983 903 3 052 913 0 619 0 135 4 232 0 631x 1 227 87 741 41 020 135 208 0 631 0 135 3 413 1 316x 1 876 26 618 9 506 45 890 1 316 0 148 7 848 0 569x 1 730 1 095 672 578 707 1 723 141 0 569 0 134 0 635 0 647x 1 726 463 125 254 604 690 453 0 647 0 136 3 812 杧 0 653x 1 358 120 665 68 001 179 114 0 653 0 135 0 601 0 395x 1 307 2 039 915 1 004 742 6 192 472 0 395 0 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