亚精胺对黄瓜幼苗灰霉病的诱抗作用

ðÚÂÛ(¼ ìç ¾h¥º FT¨尚庆茂 张志刚(ÏS jYÈ20% ,ø× H Vr50% 11 .ç ¾h )7ö<W,“ S Û(,Î VÏI N、 N0、 X、ñ、7ò、ø、 ÚT、1©¨ 3 ÿÐ 2008 M4 »19 »4 ù ChineseJournalofAppliedEcology, Apr.2008, 19(4):825-830DOI :10.13287/j.1001-9332.2008.0211200Õ±þ¥½、T L# s äF® 12 . “ -,Û(ç ¾hÄÐ j0͹ö, ÛÄÐ j0 P¨Q 、i¥9F,hð ) F9 <,ÍrT9 Û­®,ÎöÁ j0 =Ìâê ,² ¦ ËrB.' kÛ(CucumissativusL.)¹ ,ùîSpdç ¾h ) S /Û(¼ ì¥h f· 、Þ ½# FhM1þÉc ¥Y, ü SpdºÛ(¼ ì Fç ¾h¥rT# V ?¥ 3 ØÅ,¹ æ¨Äк F4Ï³ç ¾hp¸4 Ø LlG .1 ÐZE1.1 k Û( ÖÕʨÃù4|'.Spd¹SIGMA³Á Ö(Á Ö S2626,í äA8, á0.925 g·L-1,B98%), |Spd2.206 ml,¨ <£ç ¸À1 000ml ¥î2 000 mg·L-1 A,!¨.ç ¾h )® S/ =SÐý± 3þ i4.k¿2005 MÏS j< SÐý v½ñùî îj ðÑ i =É.Õ0ÑÏÕ4h,bè24h,315 °Ê4?è BÁ¥Õ0l Æ Ñ !n(9 cm×9 cm)Ï,¨¤ F¯É 13 .ÑiÌâHq¹:æ(23±3)/(12±2),1 ;v <Û ù, Ü (M A75%95%.1.2 º) Øç! k$ ,Spd !0(v)、50、100、150、200、250 mg·L-1“ i. Û uF !9, 3Qׯ, Ä) Øl u20é.46 °Û(¼ ì»2Ë=Z H,¨ÿ |Spdÿ ÆÉ( öÔÿ2 cm)Éô= Sº, Äé¨ ¹20 ml,)Ø HSpd ·Aà 1!n;2dª= ë ¨ É<ĺ, Ä) Øl u¨ (¹60ml,= ë、= ( ¨ ÒÀ ·A¸¿= ë/¯.v¨© <£9.1.3 h )¤Õ410 °,' Sºª4d¤Õç ¾h ).sY¨ )f¹0ÇA ÕZE¤Õ.)f¤Õ:¨u d u |°å¹0.4cm¥ )f¤Õ¿»2 Ë= ë, Ä Ë=P· ö=ò¤Õ1ñ )f.¨¿©çhê°å,9 Øh f· .¹0ÇA¤Õ: ¥Å¹0ÇA i¹4.3×105ñ·ml-1.¨ ¸ 9 |¹0ÇA,|±é»2 Ë= Ï À¿¹0ÇAÏÿ5 s, TVñϸ¹0ÇAÐ ð= ˤ(.¨¿¼ ì ÿ·S# 3 Ø 3Ä·S©ç.)f¹0ÇA¤Õ HÉ.¤Õ8ªì5­Â©É <Ä5 Ø,æ(21±2)/(15±1), b A90% .1.4 ©çZE1.4.1h f· sY¿¤Õª2、4、6 d¨ E3­E©çhêå、:å, Ü (´T¹hê°å,h f· 9 Ø T Â/:h f· = () Ø =òhê°å) Ø =¥hê × îµ) ØÏKvhê°å×100%1.4.2 3 Ø 3Ä·S sY¿ºª3、6、9、1215 dg |¼ ì»2 Ë=É 3 Ø 3Ä·S©ç.vMoerschbacher© 14ZE©çPAL、POD;vMiler©15ZE©çPPO;vë¡o© 16ZE©ç9p、 ËÛc ;vMorison 17ZE©ç !É Íc .1.4.3 ÿ·S 426 °,' Sºª20d,Ø°¼ ìéÚ、ÔY、= Ë 、= ë、 é7É éÄÉ . ì· =(ÔY/éÚ)× éÄÉ ×100.îµ (¨SAS ÈqDuncan¯µEÉ×1 #µsA÷s,mV (¹3Qׯ¥ Ü (´.2 ²TÐs2.1 ÷SpdÛ(¼ ìh f· ¥Y®m1 V©,ÐvM1,÷Spd üA® m1 ÷SpdÛ(¼ ìh f· ¥YFig.1 Effectsofexogenousspermidineondiseaseindexincu-cumberseedlings.:0mg·L-1Spd;:50mg·L-1 Spd;:100 mg·L-1 Spd;:150mg·L-1 Spd;:200mg·L-1 Spd;:250 mg·L-1Spd.826 ¨ 3 ÿ Ð 19 Û(¼ ì¥h f· .©ç H ù(¤Õª2、4、6d),Spd iÐh f· ïCM¥MÄ? p,' ÛSpd iïv 6Ú,h f· ïv®,®Bçñª,» Û“Spd i¥ 6Ú79F. ª üSpd®Û(¼ ìh f· i aR¥) Ø i,150mg·L-1) ØK®,¤Õª2、4、6 dh f· sY1v® 10.0%、18.5%25.4%.2.2 ÷Spdç ¾h ) S /Û(= ËÞ ½¥Y®V1 V©, Û Sºª HW¥üÉ,ò) Ø¥ Sºª6、9 d,POD ÛSpd i¥ 6Úï5®ª 6Ú¥MÄ t ; Sºª15 d, ÛSpd i¥ 6Ú,PODï5 6Úª®¥MÄ t .©ç H ù,Spdò i) Ø¥PPO(Ú¿v,SpdPPO¥9 <9VCBç¥ ir. Sºª3、6 d,Spd i®¿150mg·L-1 H, PPO ÛSpd i¥ 6Ú79 <Spd iÚ¿150 mg·L-1 H,PPO ÛSpd i¥ 6Ú7h ÐSpd i¹150 mg·L-1 H,PPOrKv´,sY1v4Ú 15.5%27.5%. Sºª9、12、15 d,PPOÚC200mg·L-1) Ø,sY1v9F 38.9%、22.6%29.7%.2.3 ÷Spdç ¾h ) S /Û(= ËÏ FhM1þÉc ¥Y®V2 V©, Û Sºª HW¥üÉ,ò)Ø9pc ï5®ª 6Ú ª û Ú £ Ü¥MÄ t .¤Õç ¾h ) -( Pºª3 d),Spdòi) Ø¥9pc (Ú¿v,9pc ÛSpdi¥ 6Ú79F,Spd i¹200mg·L-1 HrKv´,1v9F13.7%;¤Õç ¾h )ª( Pºª6、9、12、15 d),Spdò i) Ø¥9pc ïv 6Ú,°ÀÚ¿v.V1 ÷Spdç ¾h ) S /Û(¼ ì= ËÏÞ ½¥YTab.1 EffectsofexogenousspermidineonactivitiesofdefenseenzymesincucumberseedlingsleavesunderinfectionofB.cinereaSpd iSpermdineconcentration(mg·L-1) Sºª9 d,Spdò i) Ø¥ ËÛc ÐvíA÷µs; Sºª12、15 d,Spdò i) Ø¥ ËÛc (®¿v.©ç H ù,Spdò i) Ø¥ !É Íc (Ú¿v,Spd !É Íî¥ÉT¨VCBç¥ ir. Sºª3、6、9 d,Spd i®¿150 mg·L-1 H, !É Íc ÛSpd i¥ 6Ú79F,Spd iÚ¿150 mg·L-1 H, !É Íc ÛSpd i¥ 6Ú7h ,Spd i¹150 mg·L-1 H, !É Íc rKv´,sY1v4Ú 17.8%、52.0%23.6%; Sºª12、15 d, !É Íc ´C200 mg·L-1) Ø,sY1v9F18.3%44.5%.2.4 ÷Spdç ¾h ) S /Û(¼ ì 3É?â¥Y®V3 V©,÷Spdç ¾h ) S /Û(¼ ì¥ÔY、éÚ、9= ë、 é7É 、 éÄÉ、 ì· 6·S (µ üA¥ÉT¨Z 7= 9µÉT¨,ÐvM1µsA÷.SpdÛ(¼ ì 3É¥ÉT¨VC üA¥ ir,' Û i¥ïv 6Ú,Spd¼ ì¥ÉT¨ïv9 <,ò·SrKv´ª, ÛSpd i¥?à 6Ú,ÉT¨ïvh Ð.100 mg·L-1Spd) ØÛ(¼ ì 3É¥ÉT¨Kv,ÔY、9= ë、 éÄÉ 、 ì· sY1v4Ú 8.3%、35.2%、49.4%32.5%.3 ) “ -,1¿÷Spdº±þ Fh#ɱé 3É¥ùî7n¡.bÆ© 18ùîV ü,d IâHq/õ¤ ¨÷Spd Vr ìrT.' k²TV ü,÷Spd VºÛ(¼ ìç¾h¥ F,h f· 1vKÚ®25.4%;É ç ¾h ) S /Û(¼ ì¥ 3É?â,ÐvM1, ì· KÚ9F32.5%.PAL、POD、PPO ±þ FhVñÏ¥1o½. ñ ̥ڮбþ FhQµ“ Es á M¥1“,YÈ$¨T ±þ8 =ÏQ¥×1·S.PAL 6BZ ë, ñ» ?ÐXµ¥Þ ½s0²,Y ²,V7Ø« ½.'ùî²TV ü,÷Spd) تÛ(¼ ì= ËÏPAL、POD、PPOïC¥MÄ t , Û Sº HW¥üÉ,PALïv®,PODïv9 <,PPOï5®ª 6Ú®¥MÄ t .ÕMÄ t Ðv HW ûBÁ,Spd) Ø¥3Õ ½ñ k ùWB°Ú¿v £ Ü,PAL、POD、PPO3ÕÞ ½KÚsY1v9F48.4%、635.9%38.9%.yN,t ½Ð÷SpdºÛ(¼ ì Fç ¾hµBç¥M1,Û( V ?YV4Ú828 ¨ 3 ÿ Ð 19 t ½¥9 < ·Þhð ) S Æ、 ¢ü¥ ? ï.p ËþÉ# Áþ ±þ .¥ Fhy0, “·Þhðþ S ¥T¨,$ ª¹ ±þ FhÅ­B 22.9p ËÛû p ËþÉ, ÐPAL、POD、PPO á MM1. !É Í ±þ%C¥ö1îs­B,Çhð± 3þ S àpT¨,7 O !É Í®s0 p Ë -8# T¨Á3¥³ Ö VÄ )¥ 、 ½ý Í23 .' k²TV ü,¤Õç ¾h ) -( Pºª3 d),SpdÉ 9p、 ËÛ¥î¤Õç ¾h )ª, - ù( Pºª6 d)SpdÉ ËÛ¥î,7Å 9p¥î,ª ù( Pºª12、15 d)SpdÉ 9p¥î,7Å ËÛ¥î.ñ k ùW,SpdÉ !ÉÍ¥î. ª ü÷Spd V ?YVØ«Û(¼ì8 =9p、 ËÛ、 !É Í¥ 4Ú ç ¾h¥ F.N,' kÏòÕÞ ½# FhM1þÉc ¥MÄ t VCiBÁ, ½# FhM1þÉc ÚC¥ HW iµs, V ?®¿÷Spd  F|.¾ HW ¨½Ð < ¥µs ¥, s0 صÉBùî.Bî ª¹,ÂT¹»= Pбþ¥ Iâ ç|Q 24-25 .ùîV ü,ÂÄ ½(PAO)s³Â HÁ 3¥H2O2 V ?ÇÐ%Cvs0¥ ó Fhµ1,ÎÐ Ñ ùQ(HR)“d¤ F(SAR)¥|.¾Vñ 26.Shen© 27?C®Ñ ç/,Spdç) Ø VE¸± ì8ÏNADPHÄ ½¥4Ú,V7Å Ï çº¥H2O2c 6Ú,4ÚÛ( 0 Ï. 6, £Ö(SA)º¥õ¤= Ëâ ½ å º¥â ½¥ 6Úü YVSpmº¥ 28. sC.gloeosporioiddesË ª,Â) Ø VA÷®¾h𥸓 s 3¹0¥î,iÅ Í?.ÕÅrö1 ®¿ÂÅ Ca2+/CaMG |.¾Vñ 29.Kasukabe© 30?C, Æ Spdî ½cDNA¥ E 2·±é= ËÏ,Spdî ½Spdc v üA9F, H9 < Ï、ò、÷、Äl、 1i çä¤ jý© ç¥ F.cDNA± sV ü, Iâ ç/,y±éÏBty$v c, çY cy0( ÂDREB) ç ¹â( Ârd29A).V üSpd VT¹ ç|. oåÏ¥|Ø«y0,y±þ 祷 FÅ. IÓD 1 LiB-J( Ù ), FanH-Y(SZü), SunY-Q( ä l), etal.Accumulationofsalicylicacidincucum-bertreatedwithglucohexaoseanditsrelationtosystemicacquiredresistanceagainstcucumberdownymildew.Ac-taHorticulturaeSinica(óÐ), 2005, 32(1):115-117(inChinese) 2 XieB-Y(d), LiH-W( Ùò), FengL-X(§P).InductionofresistancetoPhytophthoracapsiceinpepperplantsbyDL-amino-n-bytyricacid.ActaHorticulturaeSinica(óÐ), 2002, 29(2):137-140(inChinese) 3 MartinTJ.Metablismandfunctionofpolyaminesinplants:Recentdevelopment(newapproaches).PlantGrowthRegulation, 2001, 34(1):135-148 4 WaldenR, CordeiroA, TiburcioAF.Polyamines:Smallmoleculestriggeringpathwaysinplantgrowthanddevel-opment.PlantPhysiology, 1997, 113:1009-1013 5 Kaur-SawhneyR, TibnrcioAF, GalstonAW.Spermidieandflowerbuddifferentiationinthinlayertobaccotissueculture.Planta, 1993, 173:282-284 6 MurkowskiA.Heatstressandspermidine:Effectonchlorophyllfluorescenceintomatoplants.BiologiaPlantarum, 2001, 44(1):53-577 7 MartinezMA, RamosMG, GardeaAA, etal.Effectofinfiltratedpolyaminesonpolygalacturonaseactivityandchilinginjuryresponsesinzucchinisquash(CucurbitapepoL.).BiochemicalandBiophysicalResearchCom-munications, 2002, 295(1):98-101 8 PapiyaR, KamalaN, SenGuptaDN, etal.Spermidinetreatmenttoriceseedlingsrecoverssalinitystress-in-duceddamageofplasmamembraneandPM-boundH+-ATPaseinsalt-tolerantandsalt-sensitivericecultivars.PlantScience, 2005, 168(3):583-591 9 LiG-M( Ù< ù), GuoX-L(R® ), ShangZ-Q( b), etal.AstudyofrelationshipbetweenPAsandtheresistanceoftomatotoTMV.ActaAgriculturaeBoreali-Sinica(¿ jÐ), 1998, 13(2):64-68(inChinese) 10 CohenSS, BalintR, SindhuRK.Thesynthesisofpoly-aminesfrommethionineinintactanddisruptedleafpro-toplastsofvirus-infectedChinesecabbage.PlantPhysi-ology, 1981, 68:1150-1155 11 PuL( ð á), KangZ-H( BZ), JiangJ-Z(E ), etal.SiO2 asfungicideagainstBotrytiscinereaoncucumber.ActaPhytopathologicaSinica(±þh ØÐ), 2005, 35(6):186-187(inChinese) 12 JarvisWR.Epidemiology/Coley-SmithJR, VerhoeffK, JarvisWR, eds.TheBiologyofBotrytis.London:AcademicPress, 1980 13 ShangQ-M(i ´), ChenS-F(ç Y), ZhangZ-G(f½Ð).Regulationofseleniumonantioxidativeenzymesactivityinpepperleavesunderhightemperaturestress.ActaHorticulturaeSinica(óÐ), 2005,8294 ù i ´©:ðÚÂÛ(¼ ìç ¾h¥º FT¨ 32(1):35-38(inChinese) 14 MoerschbacherBM, NolUM, FlotBE.Ligninbiosyn-theticenzymesinstemrustinfected, resistantandsus-ceptiblenear-isogenicwheatlines.PhysiologicalandMolecularPlantPathology, 1988, 33(1):33-46 15 MillerAR, KeleyTJ, MujerCV.Anodicperoxidaeisoenzymesandpolyphenoloxidaseactivityfromcucum-berfruit:Tissueandsubstratespecificity.Phytochemis-try, 1990, 29(3):705-709 16 ZhuH-B(ë¡o), TengB(- b), GaoF-L(Ú §), etal.ChangesofsomebiochemicalcharactersofsoybeanwithdiferentresistantlevelsinfectedbySMV1.ActaAgriculturaeBoreali-OccidentalisSinica( j<Ð), 2001, 10(3):38-40(inChinese) 17 MorisonIM.Asemi-micromethodfromthedetermina-tionofligninanditsuseinpredictingthedigestibilityofforagecrops.JournalofScienceFoodAgriculture, 1972,23:455-463 18 HanJ-F(bÆ), ZhuD-H(ëv), LinX-W( Ðï), etal.Functionofdiferenttreatedmethodswithpolyaminestothegrowthoftobaccoseedlings.To-baccoScience Technology(õ¤ S/), 1992(1):31-34(inChinese) 19 OuyangG-C( x;³), XueY-L(Ï C).Physi-ologicalroleandregulationofphenylpropanoidmetabo-lisminplant.PlantPhysiologyCommunications(±þ3 ØÐYß), 1988(3):9-16(inChinese) 20 DeanRA, KucJ.Rapidlignificationinresponsetowoundingandinfectionasamechanismforinducedsys-temicprotectionincucumber.PhysiologicalandMolecu-larPlantPathology, 1987, 31:69-81 21 SongF-M( à þ), ZhengZ(¢ ×), GeX-C(ë®=).ChangesofpolyphenoloxidaseactivityincotonseedlingsafterinfectionbyFusariumoxysporumf.sp.PlantPhysiologyCommunications(±þ 3 ØÐYß),1997, 33(3):175-177(inChinese) 22 LuanX-Y( k ), ChenY(ç ), DuW-G(»<), etal.Analysisofthechangesofpolyphe-nolicsubstanceinsoybeaninfectedbySMV3.SoybeanScience(vø SÐ), 2003, 22(3):186-189(inChi-nese) 23 GuoH-L(R£ ô), ChengG-W(ñôó), ChenJ(ç ®), etal.Thefunctionofphenolicmetabolisminresistantmechanismofgrayleafspotofcorn.ActaPhytopathologicaSinica(±þh ØÐ), 2003, 33(4):342-346(inChinese) 24 TassoniA, vanBurenM, FranceschettiM, etal.Poly-aminecontentandmetabolisminArabidopsisthalianaandefectofspermidineonplantdevelopment.PlantPhysiologyandBiochemistry, 2000, 38(5):383-393 25 DuanH-G( éS), HuangZ-X(ÛT), LinH-H( ¡é).Theroleofpolyaminesintheontogenyofhigherplants.ActaAgriculturaeBoreali-OccidentalisSinica( j<Ð), 2006, 15(2):190-194(inChinese) 26 WaltersD.Resistancetopathogens:Possiblerolesforfreepolyaminesandpolyaminecatabolism.NewPhytolo-gist, 2003, 159(1):109-115 27 ShenWY, NadaK, TachibanaS.Involvementofpoly-aminesinthechillingtoleranceofcucumbercultivars.PlantPhysiology, 2000, 124(1):431-439 28 TakahashiY, BerberichT, MiyazakiA, etal.Sperminesignalingintobacco:Activationofmitogen-activatedproteinkinasesbyspermineismediatedthroughmito-chondrialdysfunction.PlantJournal, 2003, 36:820-829 29 AhnIP, KimS, ChoiWB, etal.Calciumrestorespre-penetrationmorphogenesisabolishedbypolyaminesincolletotrichumgloeosporioidesinfectingredpepper.FEMSMicrobiologyLeters, 2003, 227(2):237-241 30 KasukabeY, HeL, NadaK, etal.Overexpressionofspermidinesynthaseenhancestolerancetomultipleenvi-ronmentalstressesandup-regulatestheexpressionofva-riousstress-regulatedgenesintransgenicArabidopsisthaliana.PlantandCellPhysiology, 2004, 45(6):712-722Teº i ´, 3, 1965 M 3,p V,ùîô.ö1V Yv ì ù?âØ eùî,?V Ó60Å É.E-mail:shangqmmail.caas.net.cn3 ©I f á830 ¨ 3 ÿ Ð 19