亚精胺对黄瓜幼苗灰霉病的诱抗作用

( h FT尚庆茂 张志刚(S jY20% , H Vr50% 11 . h )7W,“ S (, VI N、 N0、 X、7、 T、1 3 2008 M4 19 4 ChineseJournalofAppliedEcology, Apr.2008, 19(4):825-830DOI :10.13287/j.1001-9332.2008.0211200、T L# s F 12 . “ -,( h j0, j0 PQ 、i9F,h ) F9 ,rT9 , j0 = , rB. k(CucumissativusL.) ,Spd h ) S /( h f 、 # FhM1c Y, Spd( F hrT# V ? 3 , F4 hp4 LlG .1 ZE1.1 k ( 4|.SpdSIGMA ( S2626, A8, 0.925 gL-1,B98%), |Spd2.206 ml, 1 000ml 2 000 mgL-1 A,!. h ) S/ =S 3 i4.k2005 MS j S v j i =.04h,b24h,315 4? B0l !n(9 cm9 cm), F 13 .iHq:(233)/(122),1 ;v ; , (M A75%95%.1.2 ) ! k$ ,Spd !0(v)、50、100、150、200、250 mgL-1“ i. uF !9, 3Q, ) l u20.46 ( 2=Z H, |Spd ( 2 cm)= S, 20 ml,) HSpd A 1!n;2d= , ) l u (60ml,= 、= ( A= /.v 9.1.3 h )410 , S4d h ).sY )f0A ZE.)f:u d u |0.4cm )f2 = , =P =1 )f.h,9 h f .0A: 0A i4.3105ml-1. 9 |0A,|2 = 0A5 s, TV0A = (. S# 3 3S.)f0A H.85 5 ,(212)/(151), b A90% .1.4 ZE1.4.1h f sY2、4、6 d E3Eh、:, (Th,h f 9 T /:h f = () =h) =h ) Kvh100%1.4.2 3 3S sY3、6、9、1215 dg | 2 = 3 3S.vMoerschbacher 14ZEPAL、POD;vMiler15ZEPPO;vo 16ZE9p、 c ;vMorison 17ZE ! c .1.4.3 S 426 , S20d, 、Y、= 、= 、 7 . =(Y/) 100. (SAS qDuncanE1 #sAs,mV (3Q (.2 Ts2.1 Spd( h f Ym1 V,vM1,Spd A m1 Spd( h f YFig.1 Effectsofexogenousspermidineondiseaseindexincu-cumberseedlings.:0mgL-1Spd;:50mgL-1 Spd;:100 mgL-1 Spd;:150mgL-1 Spd;:200mgL-1 Spd;:250 mgL-1Spd.826 3 19 ( h f . H (2、4、6d),Spd ih f CMM? p, Spd iv 6,h f v,B, “Spd i 679F. Spd( h f i aR) i,150mgL-1) K,2、4、6 dh f sY1v 10.0%、18.5%25.4%.2.2 Spd h ) S /(= YV1 V, S HW,) S6、9 d,POD Spd i 65 6M t ; S15 d, Spd i 6,POD5 6M t . H ,Spd i) PPO(v,SpdPPO9 9VCB ir. S3、6 d,Spd i150mgL-1 H, PPO Spd i 679 ;Spd i150 mgL-1 H,PPO Spd i 67h ;Spd i150 mgL-1 H,PPOrKv,sY1v4 15.5%27.5%. S9、12、15 d,PPOC200mgL-1) ,sY1v9F 38.9%、22.6%29.7%.2.3 Spd h ) S /(= FhM1c YV2 V, S HW,)9pc 5 6 M t . h ) -( P3 d),Spdi) 9pc (v,9pc Spdi 679F,Spd i200mgL-1 HrKv,1v9F13.7%; h )( P6、9、12、15 d),Spd i) 9pc v 6,v.V1 Spd h ) S /( = YTab.1 EffectsofexogenousspermidineonactivitiesofdefenseenzymesincucumberseedlingsleavesunderinfectionofB.cinereaSpd iSpermdineconcentration(mgL-1) S9 d,Spd i) c vAs; S12、15 d,Spd i) c (v. H ,Spd i) ! c (v,Spd ! TVCB ir. S3、6、9 d,Spd i150 mgL-1 H, ! c Spd i 679F,Spd i150 mgL-1 H, ! c Spd i 67h ,Spd i150 mgL-1 H, ! c rKv,sY1v4 17.8%、52.0%23.6%; S12、15 d, ! c C200 mgL-1) ,sY1v9F18.3%44.5%.2.4 Spd h ) S /( 3?YV3 V,Spd h ) S /( Y、9= 、 7 、 、 6S ( AT;Z 7= 9T,vM1sA.Spd( 3TVC A ir, iv 6,Spd Tv9 ,SrKv, Spd i? 6,Tvh .100 mgL-1Spd) ( 3TKv,Y、9= 、 、 sY1v4 8.3%、35.2%、49.4%32.5%.3 ) “ -,1Spd Fh# 37n.b 18V ,d IHq/ Spd Vr rT. kTV ,Spd V( h F,h f 1vK25.4%; h ) S /( 3?,vM1, K9F32.5%.PAL、POD、PPO FhV1o. FhQ“ Es M1“,Y$T 8 =Q1S.PAL 6BZ , ?X s0,Y ,V7 .TV ,Spd) ( = PAL、POD、PPOCM t , S HW,PALv,PODv9 ,PPO5 6M t .M t v HW B,Spd) 3 k WBv ,PAL、POD、PPO3 KsY1v9F48.4%、635.9%38.9%.yN,t Spd( F hBM1,( V ?YV4828 3 19 t 9 h ) S 、 ? .p # . Fhy0, “h S T,$ FhB 22.9p p , PAL、POD、PPO MM1. ! %C1sB,h 3 S pT,7 O ! s0 p -8# T3 V ) 、 23 . kTV , h ) -( P3 d),Spd 9p、 ; h ), - ( P6 d)Spd ,7 9p, ( P12、15 d)Spd 9p,7 . k W,Spd !. Spd V ?YV(8 =9p、 、 ! 4 h F.N, k # FhM1c M t VCiB, # FhM1c C HW is, V ?Spd F|. HW s , s0 B.B ,T= P I |Q 24-25 .V , (PAO)s H 3H2O2 V ?%Cvs0 Fh1, Q(HR)“d F(SAR)|.V 26.Shen 27?C /,Spd) VE 8NADPH 4,V7 H2O2c 6,4( 0 . 6, (SA)= 6 YVSpm 28. sC.gloeosporioiddes ,) VAh“ s 30,i ?.r1 Ca2+/CaMG |.V 29.Kasukabe 30?C, Spd cDNA E 2= ,Spd Spdc v A9F, H9 、l、 1i j F.cDNA sV , I /,yBty$v c, Y cy0( DREB) ( rd29A).V Spd VT |. o|y0,y F. 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