缺磷胁迫对长豇豆幼苗脱落酸含量的影响

çÉöø¼ ì Öc ¥Y刘厚诚1 , 邝炎华2 , 陈日远1(1¿ 2 j= Râ> R½â。 ç/Éöø¼ ì ºÔ=K > Z 7=ABAc 6Ú, Ü ( 6PÖ >= Râ> R½â, ª ùABAc = Râ>PÖ > R½â ç/¼ ì >Ô=ABAc ò ÖÕû 6Ú, 6 R½â>= Râ>PÖ 。 ç/Éöø¼ ìÔ=ABAc ¥9F V ?É £s¥ 、h ÉVÄ, ç¥ aÅ­B。1oM:Éöø çABAc Ïms Ë|:Q945.1 ÓDS M :A ÓcI|:1008-505X(2004)06-0629-04Effect of phosphorus-deficiency stress on abscisic acid contents inasparagus bean seedlingsLIU Hou-cheng1 , KUANG Yan-hua2, CHEN Ri-yuan1(1 Collegeof Hort., South China Agric.Univ., Guangzhou 510642 , China ;2 College of LifeSci.,South China Agric.Univ., Guangzhou510642 , China)Abstract:Under phosphorus-deficiency stress, changes of abscisic acid (ABA)contents in seedling of three asparagusbean Vigna unquiculata W .ssp.sesquipedalis (L .)Verd cultivars differing in tolerance to phosphorus-deficiencywere studied.The results showed that ABA contents in root increased under P-deficiency stress, ABA contents declinedearly , then increased to a peak, and declined again, the peak appeared earlier in cv .Erlubai (Low P-deficiency-toler-ance)than cv .Luhuabai (High P-deficiency-tolerance).The increase in cv .Xianggangqing >cv .Erlubai >cv.Luhuabai.The ABA contents in older leaf, stem and youngest fully-expanded leaf increased under P-deficiency stress,the increase in cv.Xianggangqing >cv.Erlubai >cv .Luhuabai , but that in cv.Erlubai >cv .Xianggangqing > cv.Luhuabai at later stage .The ABA contents in younger leaf and stem increased under P-deficiency stress, the increase incv.Luhuabai >cv .Erlubai>cv .Xianggangqing.The increase of ABA contents in leaf and stem under P-deficiencystress might enhance transportation of phosphorus and water, reduce membrane lipid peroxidation .Key words:asparagus bean;phosphorus-deficiency stress;ABA contentslà ° ù:2003-09-27 ©¿à l ° ù:2003-12-18Teº: >¨ô(1968), 3,µ 2 ù÷ ¦,p V,¬ q,ö1V Y v ¡ ¡ 3 Øùî。 Ö(ABA)±þ8 = µÕÿ ?, ÂÉ= Ë , d1>,ŧè 3É,»û s ç¥ô“ûà 3É,Ø«ô“ÿ ?¥?è,i VT¹ô¹ ­W¥| T¨ 1 。¸ Ìây Í ÂÄl、e Ï、÷©HqT¨/,ABAªªá Î 6Ú,iÁB“ 3 ØMÄ,Ø«¹ ¥ 3ØîhÏ 3É, aòÕ ç。ABA |¥T¨,yN$빓 ç Í” 1 。±þ Ì⥷ F ? ï, ABA¥Q T V ?µ î。! ç/±þÁ 3¸ 3 Ø3ÄMÄ,! ç+Y ç/¥ABAMÄùî ¡。' kùî 0 ? ï¥Éöø¼ ì ç/±éòsABAc ¥MÄ,kmÙs±þ 0 ? ïÐABAQ­W¥1“。1 ÐZEkʨ 0 ñ¥Éöø ÖÕ 2 : R½â( 0 )、PÖ (ÏW Ë)、= Râ( 0 ±þ!Ðg Ð2004, 10(6):629-632Plant Nutrition and Fertilizer Science)。¨Hoagland ¥Z, !(+P) (-P)2ñ) Ø, ) Ø¥K¨K2SO4 ,¿ >¨ôi¿¥ZE 2 ¡! 。Ê |vl'BÁ¥Õ0lÕ b± ì5Ï,¨1 £ û ì5 A Î。lÕª7d ,»1= sZ 7 H, | 3ÉBÁ¥±é,Õ0=,!êô“ªM± £ ¡Q( ¸20L),±é¨ Ñ v%çç±Ï, ÄQÕ20é。!A Äï7d÷ÐBQ,¨ ,ÃY ( Ä30minY 10min)。Mª»3d 7 S Äï2d |“1Q,|“Ês¹:ô“、K > Z 7=、 ºÔ=(K> Z 7=/)、 >Ô=(K > Z 7=)。 |“ª ë'b-80bQÏ i,¨ELISAZE©ç 3 (0® 2Ø j Z 7=ABAc MÄ ç/Éöøò ÖÕ¼ ìK > Z 7=ABAc ûµMÄ(m3)。 +P) Ø R½âK >Z 7=ABAc ï® t , -PÐ+P) Ø'M », çª ùµ î 6Ú, Ü (1+P) Ø6Ú 50.80%。 +P) Ø= RâK > Z 7=ABAc ¡! - ù ÜÏ 6,»11dª 7 S/,7-P) Ø ç ùWABAc ï wLMÄ,sY»511dCÚ, Ü (1+P4Ú82.48%。+P) ØPÖ K > Z 7=ABAc ¡!5d -®, 5 7dï 6Ú,ªï®-P¥MÄÐ+P) ØM », Ü (1+P 6Ú630±þ!Ðg Ð10 88.43%。 ª ü ç/Éöø¼ ìK > Z 7=ABAc 6Ú, Ï 6Kv¥ PÖ ,Q = Râ, R½âKl, çª ù= RâABAc 1 Å Ú。m2 ç/Éöø¼ ì ºÔ=ABAc MÄFig.2 Change of ABA content in elder leaf of asparagus bean seedlings under P-deficiency stressm3 ç/Éöø¼ ìK > Z 7=ABAc MÄFig.3 Change of ABA content in youngest fully expending leaf of asparagus bean seedlings under P-deficiency stress2.4 ç/Éöø¼ ì >Ô=ABAc MÄm4 A, +P) Ø R½â >Ô=ABAc ¡! ùWï/ t , -P¥MÄÐ+P) ØM »,Ü (1+P 6Ú 62.13%。+P) Ø= Râ >Ô=ABAc 9ï/ t ,1 R½â ÜÏ,7-P) ØABAc ï wLMÄ,ÚC »7d , Ü (1+P) Ø 6Ú 53.02%。+P) ØPÖ >Ô=ABAc ïoî® t , -PÐ+P) Ø'M », Ü (1+P 6Ú 20.38%。 çÉöø¼ ì >Ô=ABAc 3 ÖÕ ( 6Ú, R½â 6Kv,PÖ Kl。m4 ç/Éöø¼ ì >=ABAc MÄFig.4 Change of ABA content in new leaf of asparagus bean seedlings under P-deficiency stress6316 ù >¨ô,©: çÉöø¼ ì Öc ¥Y3 ) µùîV ü,®¡!¥ ã½±é® £s çÁ 3 Ú¥ABAc 1Ú¡!¥±é* O Î q÷ y 4 , H d®9 y。 ç/6 =ÏABA i ±9F7ôÏí üAMÄ 5 , ±é !ÉABA¥ 9F6,=ÏABA¥³ ,®= Ë- Îq,7 OÎ ?Éô“¥ £Ðn ß Î q,9F_¹¥ £ ;±þ8 =ABA ÆÐ F I9 Z 7=ABAc 6sYPÖ = RâKÚ7 >Ô= R½âKÚ。I NABA JlM8È £Hq/¹ 3É sÅ ®¿YV Á 3 7 ;ABA i9Fµù¿»û7 Å sr KÅI N±é¹ ¥ 3É 8 ;ÐY JyI NM1,7 3I NV Y¥Á 3 Ź 3É¥ö1ðy 9 ,77 3YVr ABAsh Y¥Á 3, H9së¯ ¹ ¥ 3É 10 。 ç/Éöø¼ ìÔ=ABAc ¥9F V ?É“d© É!s £s¥ ,9 V ?h ÉVÄ,i VÅV Y¥Á 3V7»û>Ô=¥ 3É, ç¥ aÅ­B。 0¥ R½â1 0 ¥= Râ÷ ?»û >Ô=¥ 3É。ª ùABAc ¥ 6Ú V ?Ð±é¥ ºµ1,= RâABAc 1 R½âÚ, P= Râ1R½â÷¹ º,VC÷ ¨ô,i¿.Éöø ÖÕ¼ ì ç/¥ 3ÉQùî A .ÏS jÐö.ÏS M j< SÐÐ M C .Ø:ÏS j<ñ, 1999.913-917. 3ñ Á Â,ç¢o,Û. ½ ó çf%M ¸EELISA©ç =÷±þ Í J .±þ 3 ØÐYß, 1988(5):53-57. 4 Radin J W.Stomatal response to water stress and to abscisic acid inphosphorus deficient cotton plants J .Plant Physiol ., 1984, 76:392-394. 5 Jeschke W D, Peuke A U, Palo J S et al .Transport , synthesis andmetabolism of abscisic acid (ABA)in intact plant of castor bean(Rici-nus cvmmunis L .)under phosphate deficiency and moderate salinity J .J.Exp.Bot .,1997, 48:1737-1747. 6 Ù7d,Û.±þ Í# çf_©/ M . 2Ø: Ê SÐ/ ñ, 1996.124-132, 176-196. 7 Sharp R E, LeNoble M E, Else M A et al .Endogenous ABA maintainshoot growth in tomato independently of effects on plant water balance :Evidence for an interaction with ethylene J .J.Ext.Bot., 2000, 51:1575-1584. 8 Roberts J A, Hussain A ,Taylor I B, Black C R .Use of mutants to studylong-distance signaling in response to compaction strees J .J.Ext .Bot., 2002, 53:45-50. 9 Hussain A, Taylor I B, Black C R, Roberts J A .Soil compaction.A roleof ethylene in regulating leaf expansion and shoot growth in tomato J .Plant Physiol., 1999, 121:1227-1237. 10 Hussain A , Taylor I B, Black C R, Roberts J A .Does an antagonisticrelationship between ABA and ethylene mediate shoot growth when toma-to (Lycopersicon esculentum Mill.)plants encounter compacted soil J .Plant ,Cell and Envir., 2000,23:1217-1226.632±þ!Ðg Ð10