缺磷胁迫对长豇豆幼苗脱落酸含量的影响
} c Y刘厚诚1 , 邝炎华2 , 陈日远11 2 j R R。 } / K Z 7ABAc 6, 6P ] R R, ABAc RP ] R; } / ABAc 6, 6 R RP ]。 } / ABAc 9F V \ s {、h V, } aB。1oM; } ;ABAc ms |Q945.1 DS M A cI|1008-505X200406-0629-04Effect of phosphorus-deficiency stress on abscisic acid contents inasparagus bean seedlingsLIU Hou-cheng1 , KUANG Yan-hua2, CHEN Ri-yuan11 Collegeof Hort., South China Agric.Univ., Guangzhou 510642 , China ;2 College of LifeSci.,South China Agric.Univ., Guangzhou510642 , ChinaAbstractUnder phosphorus-deficiency stress, changes of abscisic acid ABAcontents in seedling of three asparagusbean [ Vigna unquiculata W .ssp.sesquipedalis L .Verd] cultivars differing in tolerance to phosphorus-deficiencywere studied.The results showed that ABA contents in root increased under P-deficiency stress, ABA contents declinedearly , then increased to a peak, and declined again, the peak appeared earlier in cv .Erlubai Low P-deficiency-toler-ancethan cv .Luhuabai High P-deficiency-tolerance.The increase in cv .Xianggangqing cv .Erlubai cv.Luhuabai.The ABA contents in older leaf, stem and youngest fully-expanded leaf increased under P-deficiency stress,the increase in cv.Xianggangqing cv.Erlubai cv .Luhuabai , but that in cv.Erlubai cv .Xianggangqing cv.Luhuabai at later stage .The ABA contents in younger leaf and stem increased under P-deficiency stress, the increase incv.Luhuabai cv .Erlubaicv .Xianggangqing.The increase of ABA contents in leaf and stem under P-deficiencystress might enhance transportation of phosphorus and water, reduce membrane lipid peroxidation .Key wordsasparagus bean;phosphorus-deficiency stress;ABA contentsl 2003-09-27 l 2003-12-18Te 1968, 3, 2 ,p V, q,1V Y v 3 。 ABA8 , \ , d1,] 3, s} “ 3,“ ,i VT W| T[ 1] 。 y l、e 、]HqT/,ABA 6,iB“ 3 M, 3h 3,[ a} 。ABA |T,yN“} ”[ 1] 。] F ], ABAQ T V ]。} / 3 3 3M,} Y } /ABAM 。 k] 0 } /sABAc M,kms 0 ABAQW1“。1 ZEk 0 ] [ 2] R 0 、P ]W 、 R 0 g 2004, 106629-632Plant Nutrition and Fertilizer Science。Hoagland Z, P -P2 , KK2SO4 , i ZE[ 2] 。 |vlB0l b 5,1 5 A 。l7d ,1 sZ 7 H, | 3B,0,“M Q 20L, v, Q20。A 7dBQ, ,Y 30minY 10min。M3d 7 S 2d |“1Q,|“s“、K Z 7、 K Z 7[/、 K Z 7[。 |“ b-80℃bQ i,ELISAZE[ 3] 0 2 j Z 7ABAc M} / K Z 7ABAc Mm3。 P RK Z 7ABAc t ], -PP M , } 6, 1P 6 50.80。 P RK Z 7ABAc - 6,11d 7 S/,7-P } WABAc wLM,sY511dC, 1P482.48。P P ]K Z 7ABAc 5d[ -, 5~ 7d 6,[;-PMP M , 1P 6630g 10 88.43。 } / K Z 7ABAc 6, 6Kv P ],Q R, RKl,} RABAc 1 。m2 } / ABAc MFig.2 Change of ABA content in elder leaf of asparagus bean seedlings under P-deficiency stressm3 } / K Z 7ABAc MFig.3 Change of ABA content in youngest fully expending leaf of asparagus bean seedlings under P-deficiency stress2.4 } / ABAc Mm4 A, P R ABAc W/ t ], -PMP M , 1P 6 62.13。P R ABAc 9/ t ],1 R ,7-P ABAc wLM,C 7d , 1P 6 53.02。P P ] ABAc o t ], -PP M , 1P 6 20.38。 } ABAc 3 6,[ R 6Kv,P ]Kl。m4 } / ABAc MFig.4 Change of ABA content in new leaf of asparagus bean seedlings under P-deficiency stress6316 , } c Y3 V , s} 3 ABAc 1* O q y[ 4] ,] H d9 y。 } /6 ABA i 9F7 AM[ 5] , ABA {9F6,ABA , - q,7 O \“ n q,9F_ ;8 ABA F I9 Z 7ABAc 6sY[P ] RK;7 [ RK。I NABA JlM8 Hq/ 3 s] YV 3[ 7] ;ABA i9F7 ] sr KI N 3[ 8] ;Y JyI NM1,7 3I NV Y 3 ] 31y[ 9] ,77 3YVr ABAsh Y 3,] H9s 3[ 10] 。 } / ABAc 9F V \{“d s s {,9 V h V,i V]V Y 3V7 3, } aB。 0 R1 0 R 3。 ABAc 6 V 1, RABAc 1 R, P R1R ,VC ,i .] } / 3Q[ A] .S j.S ] M j S M[ C] .S j, 1999.913-917.[ 3] ,o,. fM EELISA [ J] . 3 Y, 1988553-57.[ 4] Radin J W.Stomatal response to water stress and to abscisic acid inphosphorus deficient cotton plants[ J] .Plant Physiol ., 1984, 76392-394.[ 5] Jeschke W D, Peuke A U, Palo J S et al .Transport , synthesis andmetabolism of abscisic acid ABAin intact plant of castor beanRici-nus cvmmunis L .under phosphate deficiency and moderate salinity[ J] .J.Exp.Bot .,1997, 481737-1747.[ 6] 7d,. f_/ [ M] . 2 S/ , 1996.124-132, 176-196.[ 7] Sharp R E, LeNoble M E, Else M A et al .Endogenous ABA maintainshoot growth in tomato independently of effects on plant water balance Evidence for an interaction with ethylene[ J] .J.Ext.Bot., 2000, 511575-1584.[ 8] Roberts J A, Hussain A ,Taylor I B, Black C R .Use of mutants to studylong-distance signaling in response to compaction strees[ J] .J.Ext .Bot., 2002, 5345-50.[ 9] Hussain A, Taylor I B, Black C R, Roberts J A .Soil compaction.A roleof ethylene in regulating leaf expansion and shoot growth in tomato[ J] .Plant Physiol., 1999, 1211227-1237.[ 10] Hussain A , Taylor I B, Black C R, Roberts J A .Does an antagonisticrelationship between ABA and ethylene mediate shoot growth when toma-to Lycopersicon esculentum Mill.plants encounter compacted soil[ J] .Plant ,Cell and Envir., 2000,231217-1226.632g 10