缺磷胁迫对长豇豆幼苗脱落酸含量的影响
} çÉöø¼ ì Öc ¥Y刘厚诚1 , 邝炎华2 , 陈日远11¿ 2 j Râ R½â。 } ç/Éöø¼ ì ºÔK Z 7ABAc 6Ú, Ü 6PÖ ] Râ R½â, ª ùABAc RâPÖ ] R½â; } ç/¼ ì ÔABAc ò ÖÕû 6Ú, 6 R½â RâPÖ ]。 } ç/Éöø¼ ìÔABAc ¥9F V \É £s¥ {、h ÉVÄ, } ç¥ aÅB。1oMÉöø; } ç;ABAc Ïms Ë|Q945.1 ÓDS M A ÓcI|1008-505X200406-0629-04Effect of phosphorus-deficiency stress on abscisic acid contents inasparagus bean seedlingsLIU Hou-cheng1 , KUANG Yan-hua2, CHEN Ri-yuan11 Collegeof Hort., South China Agric.Univ., Guangzhou 510642 , China ;2 College of LifeSci.,South China Agric.Univ., Guangzhou510642 , ChinaAbstractUnder phosphorus-deficiency stress, changes of abscisic acid ABAcontents in seedling of three asparagusbean [ Vigna unquiculata W .ssp.sesquipedalis L .Verd] cultivars differing in tolerance to phosphorus-deficiencywere studied.The results showed that ABA contents in root increased under P-deficiency stress, ABA contents declinedearly , then increased to a peak, and declined again, the peak appeared earlier in cv .Erlubai Low P-deficiency-toler-ancethan cv .Luhuabai High P-deficiency-tolerance.The increase in cv .Xianggangqing cv .Erlubai cv.Luhuabai.The ABA contents in older leaf, stem and youngest fully-expanded leaf increased under P-deficiency stress,the increase in cv.Xianggangqing cv.Erlubai cv .Luhuabai , but that in cv.Erlubai cv .Xianggangqing cv.Luhuabai at later stage .The ABA contents in younger leaf and stem increased under P-deficiency stress, the increase incv.Luhuabai cv .Erlubaicv .Xianggangqing.The increase of ABA contents in leaf and stem under P-deficiencystress might enhance transportation of phosphorus and water, reduce membrane lipid peroxidation .Key wordsasparagus bean;phosphorus-deficiency stress;ABA contentslà ° ù2003-09-27 ©¿à l ° ù2003-12-18Teº ¨ô1968, 3,µ 2 ù÷ ¦,p V,¬ q,ö1V Y v ¡ ¡ 3 Øùî。 ÖABA±þ8 µÕÿ , Â\É Ë , d1,]ŧè 3É,»û s} ç¥ô“ûà 3É,Ø«ô“ÿ ¥è,i VT¹ô¹ W¥| T¨[ 1] 。¸ Ìây Í ÂÄl、e Ï、÷]©HqT¨/,ABAªªá Î 6Ú,iÁB“ 3 ØMÄ,Ø«¹ ¥ 3ØîhÏ 3É,[ aòÕ} ç。ABA |¥T¨,yN빓} ç Í”[ 1] 。]±þ Ì⥷ F ï], ABA¥Q T V µ î]。} ç/±þÁ 3¸ 3 Ø3ÄMÄ,} çY } ç/¥ABAMÄùî ¡。 kùî] 0 ï¥Éöø¼ ì } ç/±éòsABAc ¥MÄ,kmÙs±þ 0 ïÐABAQW¥1“。1 ÐZEkʨ 0 ñ]¥Éöø ÖÕ[ 2] R½â 0 、PÖ ]ÏW Ë、 Râ 0 ±þÐg Ð2004, 106629-632Plant Nutrition and Fertilizer Science。¨Hoagland ¥Z, P -P2ñ Ø, Ø¥K¨K2SO4 ,¿ ¨ôi ¿¥ZE[ 2] ¡ 。Ê |vl BÁ¥Õ0lÕ b± ì5Ï,¨1 £ û ì5 A Î。lÕª7d ,»1 sZ 7 H, | 3ÉBÁ¥±é,Õ0,êô“ªM± £ ¡Q ¸20L,±é¨ Ñ vçç±Ï, ÄQÕ20é。A Äï7d÷ÐBQ,¨ ,ÃY Ä30minY 10min。Mª»3d 7 S Äï2d |“1Q,|“Ês¹ô“、K Z 7、 ºÔK Z 7[/、 ÔK Z 7[。 |“ª ë b-80℃bQÏ i,¨ELISAZE©ç[ 3] 0® 2Ø j Z 7ABAc MÄ} ç/Éöøò ÖÕ¼ ìK Z 7ABAc ûµMÄm3。 P Ø R½âK Z 7ABAc ï® t ], -PÐP Ø M », } çª ùµ î 6Ú, Ü 1P Ø6Ú 50.80。 P Ø RâK Z 7ABAc ¡ - ù ÜÏ 6,»11dª 7 S/,7-P Ø } ç ùWABAc ï wLMÄ,sY»511dCÚ, Ü 1P4Ú82.48。P ØPÖ ]K Z 7ABAc ¡5d[ -®, 5~ 7dï 6Ú,[ªï®;-P¥MÄÐP ØM », Ü 1P 6Ú630±þÐg Ð10 88.43。 ª ü } ç/Éöø¼ ìK Z 7ABAc 6Ú, Ï 6Kv¥ PÖ ],Q Râ, R½âKl,} çª ù RâABAc 1 Å Ú。m2 } ç/Éöø¼ ì ºÔABAc MÄFig.2 Change of ABA content in elder leaf of asparagus bean seedlings under P-deficiency stressm3 } ç/Éöø¼ ìK Z 7ABAc MÄFig.3 Change of ABA content in youngest fully expending leaf of asparagus bean seedlings under P-deficiency stress2.4 } ç/Éöø¼ ì ÔABAc MÄm4 A, P Ø R½â ÔABAc ¡ ùWï/ t ], -P¥MÄÐP ØM »,Ü 1P 6Ú 62.13。P Ø Râ ÔABAc 9ï/ t ],1 R½â ÜÏ,7-P ØABAc ï wLMÄ,ÚC »7d , Ü 1P Ø 6Ú 53.02。P ØPÖ ] ÔABAc ïoî® t ], -PÐP Ø M », Ü 1P 6Ú 20.38。 } çÉöø¼ ì ÔABAc 3 ÖÕ 6Ú,[ R½â 6Kv,PÖ ]Kl。m4 } ç/Éöø¼ ì ABAc MÄFig.4 Change of ABA content in new leaf of asparagus bean seedlings under P-deficiency stress6316 ù ¨ô,© } çÉöø¼ ì Öc ¥Y3 µùîV ü,®¡¥ ã½±é® £s} çÁ 3 Ú¥ABAc 1Ú¡¥±é* O Î q÷ y[ 4] ,] H d®9 y。 } ç/6 ÏABA i ±9F7ôÏí üAMÄ[ 5] , ±é ÉABA¥ {9F6,ÏABA¥³ ,® Ë- Îq,7 OÎ \Éô“¥ £Ðn ß Î q,9F_¹¥ £ ;±þ8 ABA ÆÐ F I9 Z 7ABAc 6sY[PÖ ] RâKÚ;7 Ô[ R½âKÚ。I NABA JlM8È £Hq/¹ 3É s]Å ®¿YV Á 3[ 7] ;ABA i9Fµù¿»û7 ]Å sr KÅI N±é¹ ¥ 3É[ 8] ;ÐY JyI NM1,7 3I NV Y¥Á 3 ]Ź 3É¥ö1ðy[ 9] ,77 3YVr ABAsh Y¥Á 3,] H9së¯ ¹ ¥ 3É[ 10] 。 } ç/Éöø¼ ìÔABAc ¥9F V \É{“d© És £s¥ {,9 V h ÉVÄ,i V]ÅV Y¥Á 3V7»ûÔ¥ 3É, } ç¥ aÅB。 0¥ R½â1 0 ¥ Râ÷ »û Ô¥ 3É。ª ùABAc ¥ 6Ú V Ð±é¥ ºµ1, RâABAc 1 R½âÚ, P Râ1R½â÷¹ º,VC÷ ¨ô,i ¿.]Éöø ÖÕ¼ ì } ç/¥ 3ÉQùî[ A] .ÏS jÐö.ÏS ] M j SÐÐ M[ C] .ØÏS jñ, 1999.913-917.[ 3]ñ Á Â,ç¢o,Û. ½ ó çfM ¸EELISA©ç ÷±þ Í[ J] .±þ 3 ØÐYß, 1988553-57.[ 4] Radin J W.Stomatal response to water stress and to abscisic acid inphosphorus deficient cotton plants[ J] .Plant Physiol ., 1984, 76392-394.[ 5] Jeschke W D, Peuke A U, Palo J S et al .Transport , synthesis andmetabolism of abscisic acid ABAin intact plant of castor beanRici-nus cvmmunis L .under phosphate deficiency and moderate salinity[ J] .J.Exp.Bot .,1997, 481737-1747.[ 6] Ù7d,Û.±þ Í çf_©/ [ M] . 2Ø Ê SÐ/ ñ, 1996.124-132, 176-196.[ 7] Sharp R E, LeNoble M E, Else M A et al .Endogenous ABA maintainshoot growth in tomato independently of effects on plant water balance Evidence for an interaction with ethylene[ J] .J.Ext.Bot., 2000, 511575-1584.[ 8] Roberts J A, Hussain A ,Taylor I B, Black C R .Use of mutants to studylong-distance signaling in response to compaction strees[ J] .J.Ext .Bot., 2002, 5345-50.[ 9] Hussain A, Taylor I B, Black C R, Roberts J A .Soil compaction.A roleof ethylene in regulating leaf expansion and shoot growth in tomato[ J] .Plant Physiol., 1999, 1211227-1237.[ 10] Hussain A , Taylor I B, Black C R, Roberts J A .Does an antagonisticrelationship between ABA and ethylene mediate shoot growth when toma-to Lycopersicon esculentum Mill.plants encounter compacted soil[ J] .Plant ,Cell and Envir., 2000,231217-1226.632±þÐg Ð10