不同种系梅花品种组织培养繁殖研究

<p>»30 »3 ù2008 M5 Ø &nbsp; ¼“&gt; ¼“, ª üy ¼½¥F® ¡!µ“×1¥Y。2.2 9² ¡!2.2.1 梅花品种的初培养¼½º ¡!Ï Í?ª,| Í?¥Ô Vð5¥±8 Mé/ ,M Æ ¡!Ï(1 2MS ¡!)É4³,ô &nbsp; k5 ìÔ¥ %É,=Ë ÜZ、= Ë¥þ ä# M gß F®¥、þ ä#Á á Q? ¡!¥ Í ¥1。V1 3ñ ¼½ ÖÕ ¡!¥ f 。V1 3ñ ¼½ ÖÕ ¡! f TABLE 1 Thepreliminary culture situation ofthree Prunusmume cultivarsÖÕÔ %É f ôV= Ë 3É ß F®M £&#39; ®í= ËÛÄ#ÔU䵧µ Å ¦&#39; ¼Èí= Ë 3É f ȵ &#39;ȵÈ,íÛÄCµ2.2.2 增殖培养基的选择2.2.2.1 M £&#39;9²&#39; ¡!¥Ê4ùî?C,M £&#39;9² ¡!VñÏ, ¡!v í Í¥Õ Ë°¤Y k5 ì?è¥ #É, ÏQL ¡! 3É f Kz( 3É v、è¥ 、è¥ Ü (É É)¥ ¡!,7MS5 3É f Kµ¥ ¡!,Çè¥ 7 Oñ k5 ì¥9²“ ®,7M2 ¡! Ù V3É ªÐQLM1 Ú, 9²“ M ®,i O k5 ìô Û“Bç¥j ðÄ,yN975»3 ù g ö©:Õ“ ¼½ ÖÕF® ¡!N²ùîK ØX¥ ¡!(nV2)。V2 M £&#39;9²&#39; ¡!¥ ¡!²T1 TABLE 2 The comparison of culture results amongbasicproliferation media of Tie Guhong&#39;¡!è¥ è¥ Ü (É mm 3É mm9²“ QL 4.17a 9.23a 36.88a 3.23aMS 1.90c 11.95a 21.52b 1.78cWPM 2.31bc 9.75a 20.17b 2.45bcM1 2.90b 9.03a 24.27b 2.83bM2 3.72a 10.88a 37.67a 2.90bM3 2.40bc 8.99a 20.71b 1.97cÿ:B ´ª¥3 V UP =0.05 £ ܵsA÷。V3、5、6、8、9N。2.2.2.2 Å ¦&#39; ¼、 &#39;9²&#39; ¡!¥ ñÊVV3 V A, Å ¦&#39; ¼、 &#39;ò1KD¥9² ¡!sY WPM#MS ¡!, 3É f z,9´“ (Xr3 , Ö8 ¡!¥&#39;1 p。¿ Å ¦&#39; ¼7ý,MS ¡!¥9²“ dÈ®,i Oè¥ 9 &nbsp; &#39; Õ ¡!¥µs EsA÷,MS ¡!¥ 3É Ú¿WPM ¡!, 3ÁÏ Õû V¨,ZL nʨMS ¡!。V3 Å ¦&#39; ¼、 &#39;9²&#39; ¡!¥ ñÊTABLE 3 Selection of optimal media on proliferationforMeiren&#39; andYanxing&#39;ÖÕ ¡!è¥ k5 ì¥ Ü (Ú cm 3É cm9²“ Å ¦&#39; ¼MS 2.34b 2.63b 6.15b 1.48bWPM 3.25a 3.05a 10.22a 3.05a &#39; MS 3.68a 4.25a 16.64a 3.45aWPM 3.36a 4.05a 13.61a 3.32a2.2.3 铁骨红&#39;增殖培养过程中黄化问题的解决!&#39;±þF® ¡!VñÏ k5 ìÛÄ BñÜÈÛn¥Ù5,YÈ¥³ %Zõ® ¡!ÏM÷¥ i ç±þ y¹ M7Á= ËÛÄ。N, Ö8 ¡!Ï¥±þF®öÁ 3 Û?Y,7Y ¡! ¸ Ï¥ ÆöY ¡!þ¥ 3ÉsÄ,ø× HöÁ ¡!þ¥ º =。AgNO3Ï¥Ag+YVé¹²% ¥Ys8â,V7 V ÅY¥T¨。&#39;Ó Hùî M÷YÅ4b Ö i¿ÿ ¼½ k5 ìÛÄC¥rT。®V4 Vn,®i(1 £ Ü)M÷MS ¡!Ï,3ñ i¥b Ö¡!Hq/, k5 ìÛÄû Àµ¤¿ ÿ,7 Úi(2 £ Ü3 £ Ü)M÷MS ¡!Ï, i¥b Ö ¡!Hq/, ¼½ k5 ìÛÄû¤ ¿ÿ,­ b Ö2 £ Ü,&#39;AgNO3¥ i¹4mg L H k5 ì¥ÛÄCK üA。8 ²T ª¹, ¼½ k5 ìÛÄ¥ö1ðy MÁ¥,9FM÷ iª,ÛĤv¿ ÿY ÛÄ ö1ðy, b Ö iV®VÚQV YMí Í?è¿ ÿT¨。V4 iM÷AgNO3 ¼½ k5 ì 3É¥YTABLE 4 Effects of different concentrations of AgNO3 andFeon Mei flower culture in vitro¡!M÷AgNO3 i (mg·L-1)k5 ìÛÄ f &nbsp;x1 1(1MS) 1(2) 1.7 1.6 1.8 1.72 1 2(4) 2.2 2.3 2.4 2.33 1 3(8) 1.8 1.7 1.6 1.74 2(2MS) 1 3.8 3.7 3.9 3.85 2 2 4.3 4.1 4.5 4.36 2 3 3.6 3.7 3.4 3.577 3(3MS) 1 4.2 4.1 4.2 4.178 3 2 4.5 4.4 4.4 4.439 3 3 3.8 3.6 3.5 3.63K1 5.70 9.67K2 11.67 11.03K3 12.23 8.90k1 1.900 3.057k2 3.723 3.677k3 4.077 2.967R 2.177 0.710ÿ: I·S k5 ì 3É f s¹3ñ©):&#39;z、Ï、µ,sY:¹5(= Ë s 3É È)、4(= ˵ÛÄC, 3ÉÈ)、3(µÛÄ, 3ÉȵÕ=C)、2(ÛÄCø×,Õ=Cø×)、1(ÛÄø×,±é µ§)。2.2.4 碳源对铁骨红&#39;茎顶端死亡的影响÷¥1 kÏ, P¨#T¥ ¡! k5 ìC üA¥äµ§C。7 î$# ö Ñ?T¹÷¥ ¡! ,5䵧¥C¤ z¥ eÅ。yN/B¥ kÏ÷ P¨ î$ ö Ñ?, I nî&#39;y Í,Î P¨ î$T¹M £&#39; Ö8 ¡!¥÷。8 î ,M £&#39;KD¥9² 3É¥&#39; ¡!¹¿ QL ¡!,&#39;QLv í Í+P ¡!± í Í+MSµîs+2MSM÷+30 g L î$。¾ ¡!³ % F ¡ ìÛÄ、䵧©F¡Ï1 ø×¥Ù5。M £&#39;9² ìnm1。2.2.5 增殖培养基最佳激素配比的筛选2.2.5.1 M £&#39;%s ÍÕ Ë¥ ñÊQL¿ &#39; ¡!ÏF3Õ%s Í6-BA、KT、ZT, Äñ%s ÍÊ | ñ i, 3ÉÍʨNAA, i0.1 mg L。¤ª40 50 d4³ ¡!M £&#39;è¥9²rT,²TnV5。F® ¡!Ȩ¥%s ͵6-BA 、KTZT, ¼½¥9²µ¥rT。VV5 V A,3Õ%s Í ¡!¥M £&#39; k5 ì8rT6-BAZTª¿KT,7 O9²“ 9µsA÷,­ 6-BAZT iû0.5 mg L H,9²“ 76 Ø &nbsp; ¼“&gt; ¼“。&#39;ùîy ë 3ñ ¼½ ÖÕ¥ Ö8 yN8“,³ % ¼“ ÖÕM £&#39;F® ¡!VñÏÛÄ、Ô䵧¥ 45。M £&#39;KD ¡!Fî:9²¨ ¹¿ QL +1.0 mg L 6-BA +0.05 mg LNAA+0.5 mg LGA3 , 3ô¨ ¹1 2¿ QL+0.3mg L NAA +0.1 mg L IBA; Å ¦&#39; ¼KD ¡!Fî:9²¨ ¹WPM +1.0 mg L 6-BA +0.1 mg LNAA, 3ô¨ ¹1 2WPM +0.5 mg L NAA ; &#39;KD ¡!Fî:9²¨ ¹MS+0.5 mg L 6-BA+0.05mg LIBA, 3ô¨ ¹1 2MS+0.5mg LIBA。 IÓD 1ç .É.ÏS ¼½ ÖÕm½ M .Ø:ÏS &lt;ñ,1989.CHEN J Y.Mei flower cultivars of China M .Beijing:ChinaForestry PublishingHouse, 1989. 2£, g ö. ¼½© Ù ±þF® ¡!ùîC#Z¬C f U.ÏS4 óùîÉZ.Ø:ÏS &lt;ñ,2004:163-171.CAOL, L YM.Present state and progress of tissue culture inPrunus spp. C &nbsp;ZHANG Q X. Advances in ornamentalhorticulture of China.Beijing:China Forestry Publishing House,2004:163-171. 3 HARADA H, MURAI Y.Micropropagation of Prunus mume J .Plant Cell, Tissue and Organ Culture,1996, 36(4):265-267. 4 OLAYA P T, LORENZO B.Different media requirements formicropropagation of apricot cultivars J .Plant Cell, Tissue andOrgan Culture, 2000, 63(2):133-141. 5 QUOIRINM, LEPOIVRE P.Improved mediafor in vitro culture ofPrunus spp. J .Acta Horti, 1977, 78(6):437-442. 6 g ö,ç .É. ¼½L.âÕùîÉZ C &nbsp;f U.ÏS4 óùîÉZ.Ø:ÏS &lt;ñ,2004:143-148.L Y M, CHEN J Y.Genetic breeding of Mei flower (Prunusmume) C ZHANG Q X.Advances in ornamental horticulture ofChina.Beijing:China Forestry Publishing House, 2004:143-148.(责任编辑 董晓燕)79»3 ù g ö©:Õ“ ¼½ ÖÕF® ¡!N²ùî</p>