不同种系梅花品种组织培养繁殖研究

30 3 2008 M5   “> “, y F !“1Y。2.2 9 !2.2.1 梅花品种的初培养 ! ?,| ? V58 M/ ,M !(1 2MS !)4,   k5 %,= Z、= # M g F、 # Q? ! 1。V1 3 ! f 。V1 3 ! f TABLE 1 Thepreliminary culture situation ofthree Prunusmume cultivars % f V= 3 FM ' = #U ' = 3 f ',C2.2.2 增殖培养基的选择2.2.2.1 M '9' !4?C,M '9 !V, !v Y k5 ? #, QL ! 3 f Kz( 3 v、 、 ( ) !,7MS5 3 f K !, 7 O k5 9“ ,7M2 ! V3 QLM1 , 9“ M ,i O k5 “Bj ,yN9753 g :“ F !NK X !(nV2)。V2 M '9' ! !T1 TABLE 2 The comparison of culture results amongbasicproliferation media of Tie Guhong'! ( mm 3 mm9“ QL 4.17a 9.23a 36.88a 3.23aMS 1.90c 11.95a 21.52b 1.78cWPM 2.31bc 9.75a 20.17b 2.45bcM1 2.90b 9.03a 24.27b 2.83bM2 3.72a 10.88a 37.67a 2.90bM3 2.40bc 8.99a 20.71b 1.97c:B 3 V UP =0.05 sA。V3、5、6、8、9N。2.2.2.2 ' 、 '9' ! VV3 V A, ' 、 '1KD9 !sY WPM#MS !, 3 f z,9“ (Xr3 , 8 !'1 p。 ' 7,MS !9“ d,i O 9   ' !s EsA,MS ! 3 WPM !, 3 V,ZL nMS !。V3 ' 、 '9' ! TABLE 3 Selection of optimal media on proliferationforMeiren' andYanxing' ! k5 ( cm 3 cm9“ ' MS 2.34b 2.63b 6.15b 1.48bWPM 3.25a 3.05a 10.22a 3.05a ' MS 3.68a 4.25a 16.64a 3.45aWPM 3.36a 4.05a 13.61a 3.32a2.2.3 铁骨红'增殖培养过程中黄化问题的解决!'F !V k5 Bn5,Y %Z !M i y M7= 。N, 8 !F 3 ?Y,7Y ! Y ! 3s, H ! =。AgNO3Ag+YV% Ys8,V7 V YT。' H MY4b i k5 CrT。V4 Vn,i(1 )MMS !,3 ib !Hq/, k5 ,7 i(2 3 )MMS !, ib !Hq/, k5 , b 2 ,'AgNO3 i4mg L H k5 CK A。8 T , k5 1y M,9FM i,v ;Y 1y, b iVVQV YM ? T。V4 iMAgNO3 k5 3YTABLE 4 Effects of different concentrations of AgNO3 andFeon Mei flower culture in vitro!MAgNO3 i (mgL-1)k5 f  x1 1(1MS) 1(2) 1.7 1.6 1.8 1.72 1 2(4) 2.2 2.3 2.4 2.33 1 3(8) 1.8 1.7 1.6 1.74 2(2MS) 1 3.8 3.7 3.9 3.85 2 2 4.3 4.1 4.5 4.36 2 3 3.6 3.7 3.4 3.577 3(3MS) 1 4.2 4.1 4.2 4.178 3 2 4.5 4.4 4.4 4.439 3 3 3.8 3.6 3.5 3.63K1 5.70 9.67K2 11.67 11.03K3 12.23 8.90k1 1.900 3.057k2 3.723 3.677k3 4.077 2.967R 2.177 0.710: IS k5 3 f s3):'z、,sY:5(= s 3 )、4(= C, 3)、3(, 3=C)、2(C,=C)、1(, )。2.2.4 碳源对铁骨红'茎顶端死亡的影响1 k, P#T ! k5 C AC。7 $# ?T ! ,5C z e。yN/B k P $ ?, I n'y , P $TM ' 8 !。8 ,M 'KD9 3' ! QL !,'QLv +P ! +MSs+2MSM+30 g L $。 ! % F 、F1 5。M '9 nm1。2.2.5 增殖培养基最佳激素配比的筛选2.2.5.1 M '%s QL ' !F3%s 6-BA、KT、ZT, %s | i, 3NAA, i0.1 mg L。40 50 d4 !M '9rT,TnV5。F !%s 6-BA 、KTZT, 9rT。VV5 V A,3%s !M ' k5 8rT6-BAZTKT,7 O9“ 9sA, 6-BAZT i0.5 mg L H,9“ 76   “> “。'y 3 8 yN8“, % “ M 'F !V、 45。M 'KD !F:9 QL +1.0 mg L 6-BA +0.05 mg LNAA+0.5 mg LGA3 , 3 1 2 QL+0.3mg L NAA +0.1 mg L IBA; ' KD !F:9 WPM +1.0 mg L 6-BA +0.1 mg LNAA, 3 1 2WPM +0.5 mg L NAA ; 'KD !F:9 MS+0.5 mg L 6-BA+0.05mg LIBA, 3 1 2MS+0.5mg LIBA。 ID 1 .S m M .:S <,1989.CHEN J Y.Mei flower cultivars of China M .Beijing:ChinaForestry PublishingHouse, 1989. 2, g . F !C#ZC f U.S4 Z.:S <,2004:163-171.CAOL, L YM.Present state and progress of tissue culture inPrunus spp. C  ZHANG Q X. Advances in ornamentalhorticulture of China.Beijing:China Forestry Publishing House,2004:163-171. 3 HARADA H, MURAI Y.Micropropagation of Prunus mume J .Plant Cell, Tissue and Organ Culture,1996, 36(4):265-267. 4 OLAYA P T, LORENZO B.Different media requirements formicropropagation of apricot cultivars J .Plant Cell, Tissue andOrgan Culture, 2000, 63(2):133-141. 5 QUOIRINM, LEPOIVRE P.Improved mediafor in vitro culture ofPrunus spp. J .Acta Horti, 1977, 78(6):437-442. 6 g , . L.Z C  f U.S4 Z.:S <,2004:143-148.L Y M, CHEN J Y.Genetic breeding of Mei flower (Prunusmume) C ZHANG Q X.Advances in ornamental horticulture ofChina.Beijing:China Forestry Publishing House, 2004:143-148.(责任编辑 董晓燕)793 g :“ F !N