Cu胁迫对小白菜保护酶系统及其他相关抗性指标的影响

<p>y j vÐÐ(1 SÐñ)»36 »4 ùJournalofFujianAgricultureandForestryUniversity(NaturalScienceEdition) 2007 M7là ° ù:2006-06-16 &nbsp;©í ° ù:2007-03-12Á “:福建省科技厅资助项目(2005NA04).Teº:林义章(1956-),男,副教授.研究方向:蔬菜栽培生理与育种.Cu胁迫对小白菜保护酶系统及其他相关抗性指标的影响lc1,· &nbsp;Å2, 51(1.福建农林大学园艺学院,福建福州 350002;2.福建省农业厅种植业技术推广总站,福建福州 350003)K1: £ ¡!AÏFCuSO4, Z ô ¤2ñÈ?lâ ÖÕ¹ k,ùî Cu çlâ ¹ ½“d# ðM1 F·S¥Y.²TV ü:Cu çö åÊ ¹ ½“d,ÑÄþ Ä ½(SOD)、VÄ _ ½(CAT)/,VÄþ ½(POD) üA 6, HÁ 3÷¥ «VÄÁþd= (MDA), Mi9F, ¥% åÊ,7SOD/MDAc ¥® ª ü ±é¸ ç1áØ« ? ï¥/;T¹ 1iØ«þÉ,= ˳ Ö§¼ Öc Û¸Cu2+c ¥ 6ÚA÷9F.1oM:lâCu ç ½“dÏms Ë|:S634.3ÓDS M :AÓcI|:1671-5470(2007)04-0369-04EffectsofcopperstressonBrassicachinensisL.resistantenzymesystemandotherresistantindexesLINYi-zhang1, XULei2, LINBi-ying1(1.ColegeofHorticulture, FujianAgricultureandForestryUniversity, Fuzhou, Fujian350002, China;2.PlantingTechnologyExtensionStation, DepartmentofAgricultureofFujianProvince, Fuzhou, Fujian350003, China)Abstract:ThevarietiesShanghaiqingandWuyuemanwereplantedinhydroponicsolutionwithdiferentcontentsofcopper.There-sultsshowedthattheactivitiesofsuperoxidedismutase(SOD)andcatalase(CAT)wereinhibitedundercopperstress, onthecon-trarytheactivityofperoxidase(POD)andthecontentofmalondialdehyde(MDA)increased.Celmembraneintegritywasspoiledandcelmembraneleakagewasenhanced.ThedeclineofthevalueofSODactivity/MDAcontentvalueaccountedforthedeclineofself-adjustmentoftheplantundercopperstress.Asanimportantosmoregulationmaterial, freeprolineinleavessignificantlyin-creasedwithcopperconcentrationofthesolution.Keywords:BrassicachinensisL.;copperstress;enzymesystemCu Ú©±þ 3É?âVñÏA³¥BÕ×1¥± !í Í,и = ½£ ½¥FîH,CuT¹µr¥È08Ð s8,Ñ1®¥ b“#« ;T¨¥È0.¾QÏ “×1¥T¨ 1-2 .V ¥CuöÁ±þ8¥Cuý,E¸±þ¥ 3É、®Á Ö¥É . “ -áSCu±þý Ø¥ùî ¯)¿)¨ ,ö1 JTþ¹ùî, vTþ¥ùî 3-4.&#39; kÊ | ¡ ë v、Cuý ¹ ùË¥lâT¹ k ,ùîCu çlâ ¹ ½“d# ð Fy0¥Y, kmXµùî¥$ lâCuýÉÉB¥sùî,÷z¹£ UCuý¥ Ø,¹áS s ä v 3Á、Cuê ¥Í Ø4 I.1 ÐZE1.1 k klâ(BrasicachinensisL.) ÖÕ¹ Z ( ÊvÕ0µK ³)ô ¤(sÜ g ÕµK ³).1.2 &nbsp;¡!ZElâÕ0Ühý、 !ªl¿ µ Cr FÉ¥â ì ,É4-5 Ë= H,MDOI :10.13323/j.cnki.j.fafu(nat.sci.).2007.04.01550 cm×100 cm¥ Ï, ı3é×5é,ÉS!A £ ¡ 5.¨l b â ê8 ,Ä?8 2Q.1.3 &nbsp;k) Ø£ ¡) Ø7dª÷Ð ¡!A, ¡!ÏAFCuSO4 ·A.5ñ) Ø!ACu2+¥É i¹0、1、5、10、15 mg·L-1, 0mg·L-1¹v,É i0ô &nbsp;5 ùCu2+c © kªÊç. k !3ñׯ, 7-20d©çò·S.1.4 ©çZEÑÄþ Ä ½(superoxidedismutase, SOD)©ç¨ ¡ ¹:(NBT);ÄÎðE 6 ;VÄþ½(peroxidase, POD)©ç¨ß7 !pÄE 7 ;VÄ _ ½(catalase, CAT)©ç¨Ã E 8 ;d= (malondiadehyde, MDA)c ©ç¨ ;Ø1 Ö(TAB)E 9;³ Ö§¼ Öc ©ç¨Ü £ ÖE 10;M i¨,s;E©ç.2 ²TÐs2.1 Cu çlâSOD¥YV1A U Cu çlâSOD¥Y.1 mg·L-1 Cu2+) Ø¥ô ¤,SODA÷ 6Ú,ö1¿Ú©±þ8 =SOD¥3Õ ËÏ,Cu·Zn-SOD &nbsp; Kv¥1×,¸Cu¥O/,Cu·Zn-SODî9 Ód©11 ª¹,Cu ç/,POD$ 9 ¼,Û,Zò .Ü g ur ö1×Á í Í¥ê ßNJ.y j vÐÐ:1 SÐñ, 2005, 34(3):376-378. 5 õxâ.ír ¡ð ØÐ/ M.Ø:ÏS jC,f Fô.±þ F 3þÐM.Ø:ÏS jÓd, äÅ §.CuÛ(¼ ì 3É#VÄ _ ½ )Y ÖÄ ½¥YJ.±þ 3 ØÐYß, 1985(3):22-24. 12 lc, !B, ê ü. £s ç ÏÄí ù v t 3 Ø 3Ä·S¥YJ.y j&lt;vÐÐ, 1996, 25(4):438-441. 13 dë ¼, +Z C,-ü. ¥¨í ÍÐpÄ ½、ÑÄþ Ä ½¥1“J.ê &lt;vÐÐ, 2004,32(6):72-73. 14f 7 ü,Û Ê ,ðZ ,©.Cuê ¥±þý、 F Ø# ±þ©¯J. ÊÌâ S/, 2005, 18(1):4-6.(责任编辑:施晓棠) &nbsp;372y j vÐÐ(1 SÐñ)»36 </p>