Cu胁迫对小白菜保护酶系统及其他相关抗性指标的影响

pžy j v1 – S36 4 JournalofFujianAgricultureandForestryUniversityNaturalScienceEdition 2007 M7l 2006-06-16 nbsp; 2007-03-12[ “福建省科技厅资助项目2005NA04.T€e林义章1956-,男,副教授.研究方向蔬菜栽培生理与育种.Cu胁迫对小白菜保护酶系统及其他相关抗性指标的影响lc1, nbsp;2, 5‡11.福建农林大学园艺学院,福建福州 350002;2.福建省农业厅种植业技术推广总站,福建福州 350003K1 AFCuSO4,[ Z ]„ 2l’  k‹, Cu} l’  “d M1 FŸS•Y.TV Cu}    “d,  SOD、V _ CATŸ/†,V PODŸ A  6,] H 3 Vd „MDA, MiŸ9F, ›œŸž ,7SODŸ/MDAc † “} 1 /†;T 1i,   c  “Cu2c  6A9F.1oMl’;Cu} ; “dms |S634.3DS M ’AcI|1671-5470200704-0369-04EffectsofcopperstressonBrassicachinensisL.resistantenzymesystemandotherresistantindsLINYi-zhang1, XULei2, LINBi-ying11.ColegeofHorticulture, FujianAgricultureandForestryUniversity, Fuzhou, Fujian350002, China;2.PlantingTechnologyExtensionStation, DepartmentofAgricultureofFujianProvince, Fuzhou, Fujian350003, ChinaAbstractThevarietiesShanghaiqingandWuyuemanwereplantedinhydroponicsolutionwithdiferentcontentsofcopper.There-sultsshowedthattheactivitiesofsuperoxidedismutaseSODandcatalaseCATwereinhibitedundercopperstress, onthecon-trarytheactivityofperoxidasePODandthecontentofmalondialdehydeMDAincreased.Celmembraneintegritywasspoiledandcelmembraneleakagewasenhanced.ThedeclineofthueofSODactivity/MDAcontentvalueaccountedforthedeclineofself-adjustmentoftheplantundercopperstress.Asanimportantosmoregulationmaterial, freeprolineinleavessignificantlyin-creasedwithcopperconcentrationofthesolution.KeywordsBrassicachinensisL.;copperstress;enzymesystemCu  3VAB1  ,•  F;]H,CuTr08 s8,1 b“ „;†T0.Q‹ “1T[ 1-2] .ŒV Cu8Cu],E 3、†  . “ -SCu]   ,1[
JT, v’T“ [ 3-4].39; k |  v、Cu]  l’T k‹ ,Cu} l’  “d  FŸy0•Y, kmX l’Cu]›B„s,z UCu] ,S s v’ 3、Cu ™ 4• I.1 ‹ ZE1.1  k‹  kl’BrasicachinensisL.  Z ] žv0K „ s g K .1.2 nbsp;ZEl’0h、 l  Cr„ F
† ,€4-5 ‡ H,MžDOI 10.13323/ki.j.fafunat.sci..2007.04.01550 cm100 cm ž ‡, ‡35,›SA [ 5].l˜ b  8 €,8 2Q.1.3 nbsp;k  7d A, AFCuSO4 A.5 ACu2  i0、1、5、10、15 mgL-1, 0mgL-1v,  i0 nbsp;5 Cu2c  k. k 3, 7-20d[S.1.4 ZE  superoxidedismutase, SODŸ‹ NBT;E[ 6] ;Vperoxidase, PODŸ7 pE[ 7] ;V _ catalase, CATŸ E[ 8] ;d „malondiadehyde, MDAc  ;}1ˆ TABE[ 9];  c   E[ 10];M iŸ,“s;;E.2 Ts2.1 Cu} l’SODŸ•YV1A U Cu} l’SODŸ•Y.1 mgL-1 Cu2  ,SODŸA 6,ƒ18 SOD3 ˜,CuZn-SOD] nbsp; Kv1,“CuO/,CuZn-SOD†9 d[11] ,Cu} /,PODŸ 9 ,–,Z .ž g’ ur ›1 ‹  ™ N[J].žy j v1 – S, 2005, 343376-378.[ 5] x.r  / Œ[M].S jC,f F. FŸ 3[M].S jd,  .Cu 3V _ „ Y  Ÿ•Y[J]. 3 Y, 1985322-24.[ 12] lc, B, Œ . s}   ‹ v’ t 3 3S•Y[J].žy jlt;v, 1996, 254438-441.[ 13] d , Z C,-œ. žŠ p 、  Ÿ1“[J]. lt;v, 2004,32672-73.[ 14]f 7 ,  ,Z ,.Cu ™]、 FŸ  [J].  S/, 2005, 1814-6.责任编辑施晓棠 nbsp;372žy j v1 – S36 /p