Cu胁迫对小白菜保护酶系统及其他相关抗性指标的影响

y j v(1 S)36 4 JournalofFujianAgricultureandForestryUniversity(NaturalScienceEdition) 2007 M7l :2006-06-16   :2007-03-12 “:福建省科技厅资助项目(2005NA04).Te:林义章(1956-),男,副教授.研究方向:蔬菜栽培生理与育种.Cu胁迫对小白菜保护酶系统及其他相关抗性指标的影响lc1,  2, 51(1.福建农林大学园艺学院,福建福州 350002;2.福建省农业厅种植业技术推广总站,福建福州 350003)K1: !AFCuSO4, Z 2?l k, Cu l “d# M1 FSY.TV :Cu “d, (SOD)、V _ (CAT)/,V (POD) A 6, H 3 Vd= (MDA), Mi9F, % ,7SOD/MDAc 1 ? /;T 1i,= c Cu2+c 6A9F.1oM:l;Cu ; “dms |:S634.3DS M :AcI|:1671-5470(2007)04-0369-04EffectsofcopperstressonBrassicachinensisL.resistantenzymesystemandotherresistantindexesLINYi-zhang1, XULei2, LINBi-ying1(1.ColegeofHorticulture, FujianAgricultureandForestryUniversity, Fuzhou, Fujian350002, China;2.PlantingTechnologyExtensionStation, DepartmentofAgricultureofFujianProvince, Fuzhou, Fujian350003, China)Abstract:ThevarietiesShanghaiqingandWuyuemanwereplantedinhydroponicsolutionwithdiferentcontentsofcopper.There-sultsshowedthattheactivitiesofsuperoxidedismutase(SOD)andcatalase(CAT)wereinhibitedundercopperstress, onthecon-trarytheactivityofperoxidase(POD)andthecontentofmalondialdehyde(MDA)increased.Celmembraneintegritywasspoiledandcelmembraneleakagewasenhanced.ThedeclineofthevalueofSODactivity/MDAcontentvalueaccountedforthedeclineofself-adjustmentoftheplantundercopperstress.Asanimportantosmoregulationmaterial, freeprolineinleavessignificantlyin-creasedwithcopperconcentrationofthesolution.Keywords:BrassicachinensisL.;copperstress;enzymesystemCu 3?VAB1 ! , = F;H,CuTr08 s8,1 b“# ;T0.Q “1T 1-2 .V Cu8Cu,E 3、 . “ -SCu ) ,1 JT, vT 3-4.' k | v、Cu lT k ,Cu l “d# Fy0Y, kmX$ lCuBs,z UCu ,S s v 3、Cu 4 I.1 ZE1.1 k kl(BrasicachinensisL.) Z ( v0K ) (s g K ).1.2  !ZEl0h、 !l Cr F ,4-5 = H,MDOI :10.13323/j.cnki.j.fafu(nat.sci.).2007.04.01550 cm100 cm , 35,S!A 5.l b 8 ,?8 2Q.1.3  k) ) 7d !A, !AFCuSO4 A.5) !ACu2+ i0、1、5、10、15 mgL-1, 0mgL-1v, i0  5 Cu2+c k. k !3, 7-20dS.1.4 ZE (superoxidedismutase, SOD) :(NBT);E 6 ;V(peroxidase, POD)7 !pE 7 ;V _ (catalase, CAT) E 8 ;d= (malondiadehyde, MDA)c ;1 (TAB)E 9; c E 10;M i,s;E.2 Ts2.1 Cu lSODYV1A U Cu lSODY.1 mgL-1 Cu2+) ,SODA 6,18 =SOD3 ,CuZn-SOD   Kv1,CuO/,CuZn-SOD9 d11 ,Cu /,POD$ 9 ,Z . g ur 1 NJ.y j v:1 S, 2005, 34(3):376-378. 5 x.r / M.:S jC,f F. F 3M.:S jd, .Cu( 3#V _ )Y YJ. 3 Y, 1985(3):22-24. 12 lc, !B, . s v t 3 3SYJ.y j<v, 1996, 25(4):438-441. 13 d , +Z C,-. p 、 1“J. <v, 2004,32(6):72-73. 14f 7 , ,Z ,.Cu 、 F # J. S/, 2005, 18(1):4-6.(责任编辑:施晓棠)  372y j v(1 S)36