铜胁迫对小白菜叶肉细胞超微结构的影响

<p>中国生态农业学报 2008 M7 »16 »4 ùChineseJournalofEco-Agriculture,July2008, 16(4):948-951 &nbsp; DOI:10.3724/SP.J.1011.2008.00948铜胁迫对小白菜叶肉细胞超微结构的影响林义章1 张淑媛1 朱海生2(1.y j vÐóÐý Ü 350002;2.y 8 j, =、 å (m1-6)。“ Z ”)Ø i¹20 mg·L-1 H,= s8 ì ˪ ´, ìË 58 ³n, ì Ø y, ¯ V4³ Ûs¿= s8Ï,= s89 7 S? 3MÄ,®ð ¥ è oMî o(m1-9);) Ø i¹30mg·L-1 H,= s8É Ëªø× e w, ³8, = û, _Ø R ì9(m1-10);Ú ç(40 mg·L-1、50 mg·L-1) Ø/,= s8ä ê, ì ´îÀ,Ç : ¸ ˪(m1-32),= s8 =、 ³8,= s8¥É$v7ᥠ_Ø R ì &nbsp;¡,í ˪²(m1-12), O= s8X Ö 7%C,CÉCs ÖC(m1-23、m1-24)。2.4 çlâL ì8ѱ²¥Y çlâL ì8¥Y l,VmÏ ¯ ?4³“ô ¤”= ½%Ï¥L ì8 =、,Ù ¹ b &nbsp;Vn(m1 -28)。Ú) Ø/(40mg·L-1、50 mg·L-1),“ô ¤”¥L ì8 så ,ö1VC¹Ùh &gt;,ÉÏCÖ8²(m1-29),L ì8 b Ä(m1-30)。) Ø i¹20mg·L-1 H,“ Z ”= ½%Ï¥L ì8 êîÁ ál o, = C å (m1-34);) Ø i¹30mg·L-1 H,L ì8ÙM¤ y、 ´ b(m1-35);) Ø ir40mg·L-1 H,L ì8ÙÉ (,µ l oC,É s_ =o|(m1-36)。3 ) Ö0 ç %²¥ åÊ, ±þ sý¥ +, åÊñ | %¿±þ F¥ ,CÖ8², 30 U50mg·L-1) Ø¥L ì8C b Ä(27 30:×50K);31:“ Z ”10 mg·L-1) Ø¥= ½%, U= s8 ì ˪ ¹ &nbsp;(×60K);32:“ Z ”40mg·L-1) Ø¥= ½%, U= s8 ì ´îÀ,Ç : ¸ ˪(ph îU)(×40K);33 36:“ Z ”= ½%Ï¥L ì8, 33 UvL ì8,Ù b , 34¹20 mg·L-1) Ø¥L ì8, êîÁ álo, 35 U30mg·L-1) Ø,L ì8Ù y、 ´ b, 36 U40mg·L-1) Ø¥L ì8É _ =o|(ph1 î U),iµ l oC(ph2 î U)(33:×40K;34:×50K;35:×60K;36:×80K)。Chl:Chloroplast;CW:Celwal;Gra:Granum;M:Mitochondrion;P:Plasto-globulis.16:ElectronmicrographsofWuyueman&#39;smesophylcelsafectedby0mg·L-1, 10 mg·L-1, 20mg·L-1, 30mg·L-1, 40mg·L-1and50mg·L-1Custress, showthechangeofchloroplaststructure(×20K);712:ElectronmicrographsofShanghaiqingsmesophyllcellsaf-fectedby0mg·L-1, 10mg·L-1, 20mg·L-1, 30mg·L-1, 40mg·L-1, 50mg·L-1Custress, showthechangeofchloroplaststructure(×20K);1318:ElectronmicrographsofWuyueman&#39;smesophylcelsafectedby0mg·L-1, 10mg·L-1, 20mg·L-1, 30mg·L-1, 40mg·L-1, 50mg·L-1Custress, showthedecreasingcourseofchloroplastnumber(×6 000);1924:ElectronmicrographsofShanghaiqing&#39;smesophylcelsafectedby0mg·L-1, 10mg·L-1,20mg·L-1, 30mg·L-1, 40mg·L-1, 50mg·L-1Custress, showthedecreasingcourseofchloroplastnumber(×3 000);950»4 ù lc©: çlâ= ½%ѱ²¥Y25:ElectronmicrographofthemagnificationofWuyueman&#39;snormalmesophylcells, showsthegranumandstromalumen(×50K);26:Electronmi-crographofWuyueman&#39;smesophylcelafectedby30mg·L-1Custress, showsthecurvinggranumlumen, andvesicularsphereareobservedbetweenthecelwalandcytoplast(×50K);27 30:ElectronmicrographsofWuyueman&#39;smitochondria;27showsthenormalstructureofmitochondrion;28showsthestructureofmitochondrionafectedby10mg·L-1Custress;29showsthebreakdownofcristaeandappearanceofcertaincrystalstructureinmi-tochondrionwhenafectedby40mg·L-1 Custress;30 showsthevacuolizationofmitochondrionwhenafectedby50 mg·L-1 Custress(27 30:×50K);31:ElectronmicrographofShanghaiqing&#39;smesophylcelsafectedby10mg·L-1 Custress, showstheorderlychloroplastgranumlumen(×60K);32:ElectronmicrographofShanghaiqing&#39;smesophylcelsafectedby40mg·L-1Custres, showsthegranumlumenbecomeingfloc, remainessomesegment(×40K);33 36:ElectronmicrographsofShanghaiqing&#39;smitochondria;33showsthenormalstructureofmitochondrion, 34 showsthestructureofmitochondrionafectedby20mg·L-1Custress, theectoblastcondensesintocompactglobule, 35showsthestructureofmitochondrionafect-edby30mg·L-1Custress, somecristaeofmitochondriaischaos, 36showsthestructureofmitochondrionafectedby40mg·L-1Custress, plasmale-mmawrinkleandsomevesicularsphereareobserved(33:×40K;34:×50K;35:×60K;36:×80K).µ½ %ç ; 3Á ? ï¥vl。µ¡·,= s Íîh sE,ªªô Û“= s8²¥sÈ。 kV ü,® ,= s8VÈ¥õ ³nî o,Kª, =、 ï³。lâ= ½%ѱ² ç¥Qi ÖÕW¥µs,V&#39; L²T A,“ô ¤”= ½%¥= s8 =、 (Ú i ç/³h &gt;,7“ Z ”5 ç i¹30mg·L-1 H= s8 üC³8C, ª ü“ô ¤” “ Z ” 0,V7¹lâF¥y ñÊ4 Ø G &nbsp;。3.2 çlâL ì8ѱ²¥YkV ü,L ì8 ç/ ¹ 0 s¥%。L ì8= Ë ºVñÏh &gt; ,µ ¦ ª¹Ð= Ë¥« T¨µ1,= Ë ºª ù, ¯³1L ì8« 4 ? M!þÉ10 。Úi ç/,“ô ¤”= ½%ÏsL ì8Ù³h &gt;,C²Ö。Farrants©11YV²ÖÐ9 Ø£ùms/ £ L L ì8 =Ö8d8¥i,i ª¹L ì8DNAy? 3lM, VÁL ì8« &nbsp;ýÏ¥âÉVrsÈ,É7/îâÉ ½¥sÈ,VC¹Èä/ ÿòs¥Ë²Öd8+。L ì8 ØÆ ÖÖÌ 3þÄÉ¥Ê, ç/L ì8 ¥ åÊ、Ù¥h .Àh &gt;A|Y ØÆ ÖÖÌ 3þÄ¥É,V7Ylâ= Ë¥ ? 。 IÓD 1 lc,· Å.ê Ú©±þ¥ 3 ØýT¨ùîJ.ÏS 3 ÿ j¨l,¦¶ ,©.ê Ø=¤¼ ì 3É#Y¥ùîJ.¨ 3 ÿÐ, 2004, 15(1):119-122 4 lc,· Å, 5.Cu çlâ ¹ ½“d# ðM1 F·S¥YJ.y j vÐÐ,2007, 36(4):369-372 5 õxâ.ír ¡ð ØÐ/ M.Ø:ÏS j&lt;ñ, 1994:59 6 CheesemanJ.M.MechanismofsalinitytoleranceinplantsJ.PlantPhysiol., 1988, 87:547-550 7 AsadaK.Thewater-watercycleinchloroplasts:scaven-gingofactiveoxygensanddissipationofexcessphotonsJ.Annu.Rev.PlantPhysiol.PlantMol.Biol., 1999,50:601-639 8 ±Y, Ù¨, Iè,©. £= Ë¥ ºÐÑÄþ Ä ½# «VÄT¨¥1“J.±þÐ,1984, 26(6):605-615 9 JiangM.Y., YangW.Y., XuJ.Activeoxygendamageeffectofchlorophylldegrationinriceseedlingsunderos-moticstressJ.ActaBotanica, 1994, 36(4):289-295 10 CrawfordS.A., WildensS.UltrastructuralaspectsofdamagetoleavesofEucalyptuscamaldulengisbythepsyl-lidcardiaspinarotatorJ.Micron., 1996, 27:359-366 11 FarrantsG.W., HovmollerS., StadhoudersA.M.TwotypesofmitochondrialcrystalsindiseasedhumanskeletalmusclefibersJ.MuscleNerve., 1988, 11:45-55951</p>