铜胁迫对小白菜叶肉细胞超微结构的影响

中国生态农业学报 2008 M7 16 4 ChineseJournalofEco-Agriculture,July2008, 16(4):948-951   DOI:10.3724/SP.J.1011.2008.00948铜胁迫对小白菜叶肉细胞超微结构的影响林义章1 张淑媛1 朱海生2(1.y j v 350002;2.y 8 j, =、 (m1-6)。“ Z ”) i20 mgL-1 H,= s8 , 58 n, y, V4 s= s8,= s89 7 S? 3M, oM o(m1-9);) i30mgL-1 H,= s8 e w, 8, = , _ R 9(m1-10); (40 mgL-1、50 mgL-1) /,= s8 , , : (m1-32),= s8 =、 8,= s8$v7 _ R  , (m1-12), O= s8X 7%C,CCs C(m1-23、m1-24)。2.4 lL 8Y lL 8Y l,Vm ?4“ ”= %L 8 =、, b  Vn(m1 -28)。) /(40mgL-1、50 mgL-1),“ ”L 8 s ,1VCh >,C8(m1-29),L 8 b (m1-30)。) i20mgL-1 H,“ Z ”= %L 8 l o, = C (m1-34);) i30mgL-1 H,L 8M y、 b(m1-35);) ir40mgL-1 H,L 8 (, l oC, s_ =o|(m1-36)。3 ) 0 % , s +, | % F ,C8, 30 U50mgL-1) L 8C b (27 30:50K);31:“ Z ”10 mgL-1) = %, U= s8  (60K);32:“ Z ”40mgL-1) = %, U= s8 , : (ph U)(40K);33 36:“ Z ”= %L 8, 33 UvL 8, b , 3420 mgL-1) L 8, lo, 35 U30mgL-1) ,L 8 y、 b, 36 U40mgL-1) L 8 _ =o|(ph1 U),i l oC(ph2 U)(33:40K;34:50K;35:60K;36:80K)。Chl:Chloroplast;CW:Celwal;Gra:Granum;M:Mitochondrion;P:Plasto-globulis.16:ElectronmicrographsofWuyueman'smesophylcelsafectedby0mgL-1, 10 mgL-1, 20mgL-1, 30mgL-1, 40mgL-1and50mgL-1Custress, showthechangeofchloroplaststructure(20K);712:ElectronmicrographsofShanghaiqingsmesophyllcellsaf-fectedby0mgL-1, 10mgL-1, 20mgL-1, 30mgL-1, 40mgL-1, 50mgL-1Custress, showthechangeofchloroplaststructure(20K);1318:ElectronmicrographsofWuyueman'smesophylcelsafectedby0mgL-1, 10mgL-1, 20mgL-1, 30mgL-1, 40mgL-1, 50mgL-1Custress, showthedecreasingcourseofchloroplastnumber(6 000);1924:ElectronmicrographsofShanghaiqing'smesophylcelsafectedby0mgL-1, 10mgL-1,20mgL-1, 30mgL-1, 40mgL-1, 50mgL-1Custress, showthedecreasingcourseofchloroplastnumber(3 000);9504 lc: l= %Y25:ElectronmicrographofthemagnificationofWuyueman'snormalmesophylcells, showsthegranumandstromalumen(50K);26:Electronmi-crographofWuyueman'smesophylcelafectedby30mgL-1Custress, showsthecurvinggranumlumen, andvesicularsphereareobservedbetweenthecelwalandcytoplast(50K);27 30:ElectronmicrographsofWuyueman'smitochondria;27showsthenormalstructureofmitochondrion;28showsthestructureofmitochondrionafectedby10mgL-1Custress;29showsthebreakdownofcristaeandappearanceofcertaincrystalstructureinmi-tochondrionwhenafectedby40mgL-1 Custress;30 showsthevacuolizationofmitochondrionwhenafectedby50 mgL-1 Custress(27 30:50K);31:ElectronmicrographofShanghaiqing'smesophylcelsafectedby10mgL-1 Custress, showstheorderlychloroplastgranumlumen(60K);32:ElectronmicrographofShanghaiqing'smesophylcelsafectedby40mgL-1Custres, showsthegranumlumenbecomeingfloc, remainessomesegment(40K);33 36:ElectronmicrographsofShanghaiqing'smitochondria;33showsthenormalstructureofmitochondrion, 34 showsthestructureofmitochondrionafectedby20mgL-1Custress, theectoblastcondensesintocompactglobule, 35showsthestructureofmitochondrionafect-edby30mgL-1Custress, somecristaeofmitochondriaischaos, 36showsthestructureofmitochondrionafectedby40mgL-1Custress, plasmale-mmawrinkleandsomevesicularsphereareobserved(33:40K;34:50K;35:60K;36:80K). % ; 3 ? vl。,= s h sE, “= s8s。 kV , ,= s8V n o,K, =、 。l= % Qi Ws,V' LT A,“ ”= %= s8 =、 ( i /h >,7“ Z ”5 i30mgL-1 H= s8 C8C, “ ” “ Z ” 0,V7lFy 4 G  。3.2 lL 8YkV ,L 8 / 0 s%。L 8= Vh > , = T1,= , 1L 8 4 ? M!10 。i /,“ ”= %sL 8h >,C。Farrants11YV9 ms/ L L 8 =8d8i,i L 8DNAy? 3lM, VL 8  Vrs,7/ s,VC/ sd8+。L 8 3, /L 8 、h .h >A|Y 3,V7Yl= ? 。 ID 1 lc, . 3 TJ.S 3 jl, ,. = 3#YJ. 3 , 2004, 15(1):119-122 4 lc, , 5.Cu l “d# M1 FSYJ.y j v,2007, 36(4):369-372 5 x.r / M.:S j<, 1994:59 6 CheesemanJ.M.MechanismofsalinitytoleranceinplantsJ.PlantPhysiol., 1988, 87:547-550 7 AsadaK.Thewater-watercycleinchloroplasts:scaven-gingofactiveoxygensanddissipationofexcessphotonsJ.Annu.Rev.PlantPhysiol.PlantMol.Biol., 1999,50:601-639 8 Y, , I,. = # VT1“J.,1984, 26(6):605-615 9 JiangM.Y., YangW.Y., XuJ.Activeoxygendamageeffectofchlorophylldegrationinriceseedlingsunderos-moticstressJ.ActaBotanica, 1994, 36(4):289-295 10 CrawfordS.A., WildensS.UltrastructuralaspectsofdamagetoleavesofEucalyptuscamaldulengisbythepsyl-lidcardiaspinarotatorJ.Micron., 1996, 27:359-366 11 FarrantsG.W., HovmollerS., StadhoudersA.M.TwotypesofmitochondrialcrystalsindiseasedhumanskeletalmusclefibersJ.MuscleNerve., 1988, 11:45-55951