辣椒种植对根际土壤微生物多样性的影响_康林玉.pdf

2 j1 x +22 2C1,2* (1. 2v 3 H s 2 410125 2. 2 8 v 2 410125) 摘 要 Illumina MiSeq 2500 2 1“ (SJ113a06g19111) 1Z (b ) -=r 3 iM1 3sr % ) 16S rRNAy V3+V4 u ) ITS1ua“ # 3 bTV -r (M ) ) % )0 5 ) a0 ) ) ) -r % ) )F“ r % )a )M# ? 3 Mr % ) AceaChao Shannon 9F9sY 25.26%25.49%a24.51%24.87% 8.28%9.55%7 ) Shannon Simpson 9v9 78.45%275.69% r % ) ) Ts (OTU) 9 M% ) OTU - A 69 28.73%r + OTU Ah ) OTU M# M1% )vh )Mv9Fb 关 键 词 =r 3“ 中图分类号 S641.3； Q938.1+3 文献标志码 ： A 文章编号 ： 10071032(2018)02015106 Effects of pepper cultivation on the microbial diversity of rhizosphere soil KANG Linyu1 LIU Zhoubin1 OU Lijun2 TONG Hui2 PENG Ying2 YUAN Zuhua1,2* (1.Longping Branch, Graduate School of Hunan University, Changsha, Hunan 410125, China; 2.Vegetable Institution, Hunan Academy of Agricultural Science, Changsha, Hunan 410125, China) Abstract: Two pepper inbred lines, SJ113, 06g19111, and a local variety Zhangshu gang were used as materials, also using the Illumina MiSeq 2500 sequencing platform to sequencing the 16S rRNA gene V3 + V4 regions of bacteria and ITS1 region of fungal, in the rhizosphere soil before and after pepper planting. And combining the bioinformatics to analyze the richness, diversity index and community structure of the soil microorganism. The results showed that both Proteobacteria and Acidobacteria were the dominant bacteria in the soil before and after pepper cultivation. Ascomycota, Basidiomycotina and Fusarium were the dominant fungi. The bacteria species in the soil before and after the planting were more abundant and more diversified than the fungi. The relative abundance and community structure of soil bacteria and fungi had changed after pepper planting. The Ace, Chao and Shannon index of soil bacteria was increased,25.26% 25.49%, 24.51% 24.87% and 8.28% 24.87% respectively after pepper planting. The Simpson index of fungi was increased by 78.45% 78.45%, also Shannon index had a different degree of reduce after pepper planting. The number and types of OTU in soil were also changed after pepper planting, the specific OTU type in soil was reduced, while the number of bacteria OTU was significantly increased 28.73% after pepper planting. The effect of variety difference on OTU type of fungi was greater than that of bacteria, and the relative abundance of pathogenic fungi was significantly increased. Keywords: pepper; rhizosphere soil; microbial diversity 收稿日期 20170623 修回日期 20170913 基金项目 SE S/9 “ (2014BAD05B04) 作者简介 B (1993i) om V 31V Y 3a8T 18874923457163.com *YTC1V Y v 3 3#s0 3 csyzh888126.com 152 2 jr V “% ) ”_ “ ) ”r b k -r % ) AceaChao Shannon (A ) 7 Simpson 5A )bV -r % ) )F“ r B “% ) ”r b H % )A 6“9 9F7 ) AM f/ “ b CARTWRIGHT13 FIERER 14r 3“ “M1TBb/My V ? -r $ 3 3 Yb kr % ) ) OTU “ Ace Chao M? pB% ) OTU is% ) ) OTU 9 OTU vs-r c + OTU 7 r + OTU Ah O W ) OTU s v% )bV r 3 F? 3 AMbM V ? 3?V 3“s r 3Y15169V ? r 3YVM“s s W e= 3 1718b“s sr ) WM $u / + )v41920b k ) a ) ) h )v9Fb - 219V ) ) )h h )7 h ) 8v 9K 3?h . yN Pr % )F “% ) ”r H/ )“h Ov9Fh )Mb 参考文献： 1 k WyZ 2Pr 3r y01“ J T 2010 31(4)530535. 2 f w = r m 3 u$ r 3+ J 3 201021(1) 165173. 3 W 3 | O r a!s 3M1 JS 3 j S; ZE : 3 #rTY J J S 2013 34(14)346350 12 :F/ D =Sv 2008 13 ( 71o/ Ds j v 2015 14 :f + 4 7 J 7F 2010 10(3) 3032 15 : f Z T :# O Y J J :s# FY J a1v 2011 32(2)172175 22 v 7/ #Ds j v 2013 23 r 6r B T Y J J S 2012 33(16) 314317 24 b s Y JL jk W j Z T MY J 7 2005(4) 3234 26 bBW : Y J j . T r 3“Y J.S 3 j 2015 23(2) 225232. 13 CARTWRIGHT J DZANTOR E K MOMEN B Soil microbial community profiles and functional diversity in limestone cedar gladesJ Catena 2016 147 216224. 14 FIERER N JACKSON R B. The diversity and biogeography of soil bacterial communitiesJ. Proceedings of the National Academy of Sciences of the United States of America2006 103(3) 626631. 15 uM S L 3 “s Fss J j 2012 21(8)175181. 16 BRENNAN E B ACOSTAMARTINEZ V Cover cropping frequency is the main driver of soil microbial changes during six years of organic vegetable productionJ. Soil Biology and Biochemistry 2017 109188204. 17 MARSCHNER P CROWLEY D E HIGASHI R M Root exudation and physiological status of a rootcolonizing fluorescent pseudomonad in mycorrhizal and non mycorrhizal pepper (Capsicum annuum L.)J Plant and Soil1997 189(1) 1120. 18 _ “s / r 3T1“ZZ J 3 2014 38(3) 298310. 19 CHAPARRO J M BADRI D V VIVANCO J M. Rhizosphere microbiome assemblage is affected by plant developmentJ The ISME Journal 2014 8(4) 790803. 20 Z d1S K= 3 # M Jr 2005 42(3)488494. 21 qf0zh )s k JS S 2014(5) 8488 责任编辑：毛友纯 英文编辑：雷东阳